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HRP anti-β-Amyloid, 1-40 Antibody (Previously Covance catalog# SIG-39146)

Pricing & Availability
Clone
11A50-B10 (See other available formats)
Regulatory Status
RUO
Other Names
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously
Signet Catalog# 9146-02
Covance Catalog# SIG-39146
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
11A50-B10_HRP_betaAmyloid_1-40_1_091019
IHC staining of HRP anti-β-Amyloid, 1-40 antibody (clone 11A50-B10) on formalin-fixed paraffin-embedded Alzheimer’s disease human brain tissue. Following antigen retrieval using 88% formic acid for 20 minutes at room temperature, the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 11A50-B10_HRP_betaAmyloid_1-40_1_091019
    IHC staining of HRP anti-β-Amyloid, 1-40 antibody (clone 11A50-B10) on formalin-fixed paraffin-embedded Alzheimer’s disease human brain tissue. Following antigen retrieval using 88% formic acid for 20 minutes at room temperature, the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 11A50-B10_HRP_betaAmyloid_1-40_2_091019
    IHC staining of HRP anti-β-Amyloid, 1-40 antibody (clone 11A50-B10) on formalin-fixed paraffin-embedded Alzheimer’s disease human brain tissue. Following antigen retrieval using 88% formic acid for 20 minutes at room temperature, the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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805407 200 µL 507€
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Description

Amyloid beta (Aβ or Amyloid beta) denotes peptides of 36-43 amino acids in length that are crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. The peptides result from the amyloid precursor protein (APP), which is cut by certain enzymes to yield Aβ. Aβ molecules can aggregate to form oligomers (known as "seeds") which are believed to be able to induce other Aβ molecules to also take the misfolded oligomeric form, leading to a chain reaction akin to a prion infection. The seeds or the resulting amyloid plaques are toxic to nerve cells. The other protein implicated in Alzheimer's disease, tau protein, also forms such prion-like misfolded oligomers, and there is some evidence that misfolded Aβ can induce tau to misfold.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Aqueous buffered solution containing a carrier protein and preservatives.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store between 2-8°C. Do not store antibody diluted below 50 ug/mL in the absence of protein (i.e.: add 2% bovine serum albumin).
Application

IHC-P - Quality tested
Dot Blot, ELISA, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 11A50-B10 is reactive to the C-terminus of beta amyloid and is specific for the isoform ending at the 40th amino acid.

Application References
  1. Watts JC, et al. 2014. Proc. Natl. Sci. USA 28:10323. (IHC-P) PubMed
  2. Diomede L, et al. 2014. Neurobiol. Dis. 62:521. (Dot Blot) PubMed
  3. Watanabe H, et al. 2012. J Neurosci. 15:5085. (ELISA) PubMed
  4. Portelius E, et al. 2006. J. Proteome Res. 4:1010. (IP)
  5. Vingtdeux V, et al. 2011. FASEB J. 25:219. (ELISA) PubMed
  6. Ohmi K, et al. 2011. PLoS One 11:e27461. (IHC-Other) PubMed
Product Citations
  1. Casali BT, et al. 2020. Neurobiol Dis. 142:104956. PubMed
  2. Casali BT, et al. 2018. J Neuroinflammation. 15:43. PubMed
  3. Cheng-Hathaway PJ, et al. 2018. Mol Neurodegener. 13:e0200060. PubMed
RRID
AB_2564979 (BioLegend Cat. No. 805407)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid 1-40 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All β-Amyloid, 1-40 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-β-Amyloid, 1-40 11A50-B10 ELISA,WB,IHC,IP
HRP anti-β-Amyloid, 1-40 11A50-B10 IHC-P,Dot Blot,ELISA,IP
Purified anti-β-Amyloid, 1-40 11A50-B10 IHC-P,Dot Blot,ELISA
Go To Top Version: 5    Revision Date: 09-10-2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-β-Amyloid, 1-40

  • HRP anti-β-Amyloid, 1-40

  • Purified anti-β-Amyloid, 1-40

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