Go-ChIP-Grade™ Purified Mouse IgG2b, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
MG2b-57 (See other available formats)
Isotype
Mouse IgG2b, κ
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Product Citations
publications
MG2b-57_GoChIP_IgG2b-k_ctrl_1_060618_updated
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HaCaT cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 (clone W15093A, Cat. No. 687203) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2b, κ isotype control antibody (clone MG2b-57, Cat. No. 401213) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • MG2b-57_GoChIP_IgG2b-k_ctrl_1_060618_updated
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HaCaT cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 (clone W15093A, Cat. No. 687203) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2b, κ isotype control antibody (clone MG2b-57, Cat. No. 401213) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • MG2b-57_GoChIP_IgG2b-k_ctrl_2_060618_updated
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HaCaT cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 (clone W15093A, Cat. No. 687203) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2b, κ isotype control antibody (clone MG2b-57, Cat. No. 401213) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human MDM2 gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
Cat # Size Price Quantity Avail. Save
401213 25 µg 24€
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401214 100 µg 68€
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Description

The isotype of MG2b-57 immunoglobulin is mouse IgG2b,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Trinitrophenol + KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
FC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by ChIP assay as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.

Application Notes

The MG2b-57 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. Nos. 401215-401220) as negative control.

Application References
  1. Magatti M, et al. 2008. Stem Cells 26:182. (FC) PubMed

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Go To Top Version: 1    Revision Date: 05-23-2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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