- Clone
- 12A4A35 (See other available formats)
- Other Names
- Interferon regulatory factor-3 (IRF-3)
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Chromatin Immunoprecipitation (ChIP) was performed using Go-ChIP-Grade™ Protein G Enzymatic Kit by loading 3 µg of cross-linked chromatin samples from THP-1 cells treated with LPS with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-IRF3 (Clone 12A4A35), or B) equal amount of Purified Mouse IgG2b, κ Isotype Control Antibody. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human IFNB gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin. -
Total lysates (15 µg protein) from Daudi (lane 1) and A20 (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-IRF3 antibody (upper). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody or HRP Donkey anti-rabbit IgG Antibody for GAPDH and chemiluminescence detection. GAPDH (poly6314) antibody was used as a loading control (lower). Lane M: Molecular weight ladder. -
Jurkat cells were stained with purified anti-IRF3 (12A4A35) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody. Nuclei were stained with DAPI (blue).
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 655703 | 25 µg | 60€ | ||||
| 655704 | 100 µg | 188€ | ||||
IRF-3 is a member of the interferon regulatory transcription factor (IRF) family. It mediates interferon-stimulated response element (ISRE) promoter activation and functions as a molecular switch for antiviral activity. DsRNA generated during the course of a viral infection leads to IRF-3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB protein binding to dsRNA-activated factor 1, a complex which activates the transcription of genes under control of ISRE. The phosphorylated IRF-3 is under negative regulation by ubiquitin-mediated degradation. Research indicates that it plays a critical role in stimulated TLR-mediated IL-27 p28 gene expression.
Product DetailsProduct Details
- Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial IRF-3 recombinant protein (200-427 a.a.)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ChIP - Quality tested
IF, WB - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50-1:100 by volume. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent to be titrated for optimal performance before each experiment.
- Application Notes
-
25 µg, 100 µg of Go-ChIP-Grade™ Purified Antibody can be used for 2-4, 11-16 immunoprecipitations, respectively, at the recommended dilution.
- RRID
-
AB_2721589 (BioLegend Cat. No. 655703)
AB_2721590 (BioLegend Cat. No. 655704)
Antigen Details
- Structure
- 427 amino acids, predicted molecular weight of 47 kD. Contains an IRF tryptophan pentad repeat responsible for DNA binding.
- Distribution
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Inactive IRF-3 continuously transits between the cytoplasm and nucleus. Serine/threonine phosphorylation or IRF-3 results in the structural changes which allow IRF-3 activation. The activated IRF3 translocates to the nucleus, where it forms a complex with CREB binding protein and activates the transcription of interferons, interferon stimulated genes, and other antiviral response genes.
- Function
- IRF-3 is a constitutively expressed, latent transcription factor that plays a pivotal role in type I IFN response.
- Interaction
- CREB binding protein, IRF-7, TICAM-1, TICAM-2, non-canonical IKB kinases TBK1 and IKKε.
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Lin R, et al. 1999. Mol. Cell Biol. 19:959.
2. Hiscott J, et al. 2007. J. Biol. Chem. 282:15325.
3. Molle C, et al. 2010. J. Immunol. 184:1784. - Gene ID
- 3661 View all products for this Gene ID
- UniProt
- View information about IRF3 on UniProt.org
Related Pages & Pathways
Pathways
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