Go-ChIP-Grade™ Purified anti-Histone H3 (C-terminus) Antibody

Pricing & Availability
Clone
1B1-B2 (See other available formats)
Other Names
Histone H3.1, histone H3/a, histone 1, H3a, H3 histone family, member A, H3/A, H3FA
Isotype
Mouse IgG3
Ave. Rating
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Product Citations
publications
1_1B1-B2_GoChIP_HistoneH3_Antibody_050918
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-Histone H3 (C-terminus) (Clone 1B1-B2, Cat. No. 819403) or B) equal amount of Purified Mouse IgG3 Isotype Control Antibody (Clone MG3-35, Cat. No. 401301) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human GAPDH gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 1_1B1-B2_GoChIP_HistoneH3_Antibody_050918
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-Histone H3 (C-terminus) (Clone 1B1-B2, Cat. No. 819403) or B) equal amount of Purified Mouse IgG3 Isotype Control Antibody (Clone MG3-35, Cat. No. 401301) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human GAPDH gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 2_1B1-B2_PURE_Histone_H3_Antibody_WB_011618
    Total lysates (15 µg protein) from HeLa (lane1), Raw 264.7 (lane 2) and UMR106 (lane 3) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:50,000 diluted (0.01 µg/mL) Purified anti-Histone H3 Antibody, clone 1B1-B2 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted anti-Mouse-IgG secondary antibody conjugated to HRP for the anti-Histone H3 Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin Antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder.
  • 3_1B1-B2_PURE_Histone_H3_Antibody_IHC_050918
    IHC staining of purified anti-Histone H3 (C-Terminus) antibody (clone 1B1-B2) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
Cat # Size Price Quantity Avail. Save
819403 25 µg 60€
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819404 100 µg 188€
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Description

Histone proteins are classified into core histones (H2A, H2B, H3, H4) and linker histones (H1, H5). Core histones form an octamer, which contains two H2A-H2B dimers and one H3-H4 tetramer. Core histones are predominantly globular except for the unstructured N-terminal tails. Posttranslational modifications, such as acetylation, methylation, phosphorylation, ubiquitination, SUMOylation and ADP-ribosylation occur in histone tails.

Histone modifications induce changes of chromatin structure and thereby affect the accessibility of transcription factors, nuclear proteins and enzymes to genomic DNA, resulting in gene activation or repression. It is known that histone modifications play critical roles in DNA repair, DNA replication, transcription regulation, alternative splicing and chromosome condensation and some diseases including autoimmune diseases and cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a peptide sequence derived from the C-terminal domain of human Histone H3 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
IHC-P, WB - Validated 

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50-1:150 by volume. For immunohistochemistry, a dilution of 1:1000 is suggested. For Western blotting, the suggested use of this reagent is 0.01 µg/mL (1:50000). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Reactivity to Drosophila was only validated with the purified format.

Additional Product Notes

25 µg and 100 µg of Go-ChIP-Grade™ Purified Antibody can be used for 2-7 or 10-30 immunoprecipitations, respectively, at the recommended dilutions.

RRID
AB_2650676 (BioLegend Cat. No. 819403)
AB_2650662 (BioLegend Cat. No. 819404)

Antigen Details

Structure
Histone proteins H3 and H4 bind to form a H3-H4 dimer, two of these H3-H4 dimers combine to form a tetramer. This tetramer further combines with two H2a-H2b dimers to form the compact Histone octamer core.
Distribution

Nucleus.

Function
Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Interaction
Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Antigen References

1. Chen HM, et al. 2015. Epigenetics and Dermatology 409.

Gene ID
8350 View all products for this Gene ID
UniProt
View information about Histone H3 (C-terminus) on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 10-03-2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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