- 2F5A22 (See other available formats)
- Other Names
- REL, V-Rel Avian Reticuloendotheliosis Viral Oncogene Homolog, Proto-Oncogene C-Rel
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
|Cat #||Size||Price||Quantity Avail.||Save|
c-Rel is a member of the Rel/NF-κB transcription factor family that forms homo or heterodimers with family members such as RelA/p65, RelB, NF-κB1/p105, and NF-κB2/p100. The NF-κB complex is retained in cytoplasm by the inhibitor IκB protein in unstimulated cells. Upon activation, IκB is degraded via the ubiquitin-proteasome pathway, which results in nuclear translocation of the active NF-κB complex. While other NF-κB transcription factors are expressed in most cell types, relatively higher levels of c-REL expression are found in hematopoietic cells. c-REL is thought to play key roles in regulating proliferation, differentiation, and survival of B and T lymphocytes.Product Details
- Antibody Type
- Host Species
- Partial c-REL recombinant protein (453-587 a.a.)
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
ChIP - Quality tested
WB - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50-1:100 by volume. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml.
It is recommended that the reagent to be titrated for optimal performance before each experiment.
- Application Notes
25 µg, 100 µg of Go-ChIP-GradeTM Purified Antibody can be used for 2-4, 11-16 immunoprecipitations, respectively, at the recommended dilution.
AB_2687119 (BioLegend Cat. No. 655803)
AB_2687120 (BioLegend Cat. No. 655804)
- 619 amino acids, predicted molecular weight of 69 kD, contains a RHD (Rel-like) domain responsible for dimerization and DNA binding, contains two C-terminal transactivation domains
Cytoplasm, translocated to the nucleus upon activation
- Transcription factor belonging to the NF-κB family, regulates various biological processes including tumorgenesis, inflamation, immune response, cell proliferation, and differentiation
- Forms homo or heterodimer with other NF-κB family members; interacts with NKIRAS1, NFKBIB, NFKBIE, IRF-8, and Foxp3
- Cell Type
- B cells, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Signal Transduction, Transcription Factors
- Molecular Family
- TCRs, Nuclear Markers
- Antigen References
1. Bash J, et al. 1997. Mol. Cell Biol. 17:6526.
2. Chen G, et al. 2010. J. Immunol. 185:2350.
3. Gilmore TD, et al. 2011. Genes Cancer 2:695.
4. Ferch U, et al. 2007. Nat. Immunol. 8:984.
5. Sanjabi S, et al. 2005. Genes Dev. 19:2138.
6. Yamazaki T, et al. 2003. Nat. Immunol. 4:780.
- Gene ID
- 5966 View all products for this Gene ID
- View information about c-REL on UniProt.org
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.