Go-ChIP-Grade™ Purified anti-c-REL Antibody

Pricing & Availability
Clone
2F5A22 (See other available formats)
Other Names
REL, V-Rel Avian Reticuloendotheliosis Viral Oncogene Homolog, Proto-Oncogene C-Rel
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
2F5A22_GoChIP_c-REL_Antibody_042617
Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3µg of cross-linked chromatin samples from Jurkat cells treated with TPA and Ionomycin with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-c-REL (Clone 2F5A22), B) equal amount of Purified Mouse IgG2b, κ Isotype Control Antibody, or C) competitor’s ChIP-grade Purified anti-c-REL Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human Bcl2L1 gene region and human αSatellite repeat (as a negative control). The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • 2F5A22_GoChIP_c-REL_Antibody_042617
    Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3µg of cross-linked chromatin samples from Jurkat cells treated with TPA and Ionomycin with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-c-REL (Clone 2F5A22), B) equal amount of Purified Mouse IgG2b, κ Isotype Control Antibody, or C) competitor’s ChIP-grade Purified anti-c-REL Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human Bcl2L1 gene region and human αSatellite repeat (as a negative control). The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • 2F5A22_GoChIP_cREL_Antibody_WB_042717
    Total lysates (15 µg protein) from HeLa (lane 1), Daudi (lane 2), and NIH3T3 (lane 3) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-c-REL Antibody, clone 2F5A22 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-c-REL Antibody. 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody (2F1-1) was used as a loading control (lower). Lane M: Molecular weight ladder.
Cat # Size Price Quantity Avail. Save
655803 25 µg 60€
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655804 100 µg 188€
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Description

c-Rel is a member of the Rel/NF-κB transcription factor family that forms homo or heterodimers with family members such as RelA/p65, RelB, NF-κB1/p105, and NF-κB2/p100. The NF-κB complex is retained in cytoplasm by the inhibitor IκB protein in unstimulated cells. Upon activation, IκB is degraded via the ubiquitin-proteasome pathway, which results in nuclear translocation of the active NF-κB complex. While other NF-κB transcription factors are expressed in most cell types, relatively higher levels of c-REL expression are found in hematopoietic cells. c-REL is thought to play key roles in regulating proliferation, differentiation, and survival of B and T lymphocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial c-REL recombinant protein (453-587 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
WB - Verified 

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50-1:100 by volume. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml.

It is recommended that the reagent to be titrated for optimal performance before each experiment.

Application Notes

25 µg, 100 µg of Go-ChIP-GradeTM Purified Antibody can be used for 2-4, 11-16 immunoprecipitations, respectively, at the recommended dilution.

RRID
AB_2687119 (BioLegend Cat. No. 655803)
AB_2687120 (BioLegend Cat. No. 655804)

Antigen Details

Structure
619 amino acids, predicted molecular weight of 69 kD, contains a RHD (Rel-like) domain responsible for dimerization and DNA binding, contains two C-terminal transactivation domains
Distribution

Cytoplasm, translocated to the nucleus upon activation

Function
Transcription factor belonging to the NF-κB family, regulates various biological processes including tumorgenesis, inflamation, immune response, cell proliferation, and differentiation
Interaction
Forms homo or heterodimer with other NF-κB family members; interacts with NKIRAS1, NFKBIB, NFKBIE, IRF-8, and Foxp3
Cell Type
B cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Signal Transduction, Transcription Factors
Molecular Family
TCRs, Nuclear Markers
Antigen References

1. Bash J, et al. 1997. Mol. Cell Biol. 17:6526.
2. Chen G, et al. 2010. J. Immunol. 185:2350. 
3. Gilmore TD, et al. 2011. Genes Cancer 2:695. 
4. Ferch U, et al. 2007. Nat. Immunol. 8:984. 
5. Sanjabi S, et al. 2005. Genes Dev. 19:2138.
6. Yamazaki T, et al. 2003. Nat. Immunol. 4:780.

Gene ID
5966 View all products for this Gene ID
UniProt
View information about c-REL on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 10-30-2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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