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FITC anti-mouse CD199 (CCR9) Antibody

Pricing & Availability
Clone
9B1 (See other available formats)
Regulatory Status
RUO
Other Names
GPR 9-6, CDw199, Chemokine Receptor 9
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
9B1_FITC_100908.jpg
C57BL/6 thymocytes stained with 9B1 FITC
  • 9B1_FITC_100908.jpg
    C57BL/6 thymocytes stained with 9B1 FITC
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129705 50 µg 95€
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129706 500 µg 292€
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Description

CCR9 is a member of the G protein coupled receptor family and is involved in T cell development in the thymus and in gut-associated immune response. Mouse CCR9 is highly expressed on different stages of thymocytes and upregulated on CD4+CD8+ cells. It is expressed to a much lower level on spleen and lymph node cells. Expression of CCR9 on γ/δ T cells in the intraepithelial and small intestine has been reported. The interaction of CCR9 with its ligand CCL25 (TECK, thymus-expressed chemokine) may direct the trafficking of developing T cells in the thymus and generation of gut-specific immunological memory.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Blue Laser (488 nm)
Product Citations
  1. Flamar AL, et al. 2020. Immunity. 52(4):606-619.e6.. PubMed
  2. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  3. Highton AJ, et al. 2019. Wellcome Open Res. 4:78. PubMed
RRID
AB_1227482 (BioLegend Cat. No. 129705)
AB_1227483 (BioLegend Cat. No. 129706)

Antigen Details

Structure
A membrane of G-protein coupled receptors and contains seven transmembrain domains. Mouse CCR9 has above 80% amino acid sequence homology with human counterpart.
Distribution
CD4+CD8+ thymocytes, γ/δ T cells
Interaction
CCL25
Ligand/Receptor
CCL25
Cell Type
T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Zaballos A, et al. 1999. J. Immunol. 162:5671
2. Wurbel MA, et al. 2007. J. Immunol. 178:7598
3. Wurbel MA, et al. 2006. Eur. J. Immunol. 36:73

Gene ID
12769 View all products for this Gene ID
UniProt
View information about CD199 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 2    Revision Date: 02-18-2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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