- MIH3 (See other available formats)
- Regulatory Status
- Other Names
- Programmed cell death ligand 1 (PD-L1), B7 homolog 1 (B7-H1), B7-H, B7H1, PDL1, PDCD1L1, PDCD1LG1
- Mouse IgG1, κ
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- Product Citations
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CD274, also known as PD-L1 and B7-H1, is type I transmembrane glycoprotein that serves as a ligand for CD279 (PD-1). This interaction is believed to regulate the balance between the stimulatory and inhibitory signals needed for responses to microbes and maintenance of self-tolerance. CD274 is involved in the costimulation of T cell proliferation and IL-10 and IFN-γ production in an IL-2-dependent and CD279-independent manner. Conflicting data has shown that CD274 can inhibit T cell proliferation and cytokine production, and alternatively, enhance T cell activation. Other studies suggest that CD274 may signal bidirectionally, raising interesting implications for its expression in a wide variety of cell types, including T and B cells, antigen-presenting cells, and non-hematopoietic cells.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Human PD-L1-transfected cells.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include blocking1, 2.
Clone MIH3 blocks the binding of PD-L1 with PD-1 , but did not block the binding of PD-L1 with B7-1 (CD80).
- Application References
- Haile S.T, et al. 2014. Cancer Immunol Res. 2:1. (Block)
- Butte M.J, et al. 2008. Mol Immunol. 45:3567. (Block)
AB_2734437 (BioLegend Cat. No. 374509)
AB_2734438 (BioLegend Cat. No. 374510)
- Type 1 transmembrane protein, member of the B7 family, 40kD.
T cells, B cells, NK cells, monocytes/macrophages, granulocytes and dendritic cells.
- CD274 is involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-CD1-independent manner. Its interaction with PD-1-CD1 inhibits T-cell proliferation and cytokine production.
- PD-1 (CD279), B7-1 (CD80).
- Cell Type
- T cells, B cells, NK cells, Monocytes, Macrophages, Granulocytes, Dendritic cells
- Biology Area
- Immunology, Cancer Biomarkers, Costimulatory Molecules
- Molecular Family
- Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
- Antigen References
1. Khan AR, et al. 2015. Nat Commun. 6:5997.
2. Kiyasu J, et al. 2015. Blood. 126:2193.
3. Herold M, et al. 2015 J Immunol. 195:3584.
4. Buddhisa S, et al. 2015. J Immunol. 194:4413.
- Gene ID
- 29126 View all products for this Gene ID
- View information about CD274 on UniProt.org
|Ultra-LEAF™ Purified anti-human CD274 (B7-H1, PD-L1)||MIH3||FC, Block|
|PE/Cyanine7 anti-human CD274 (B7-H1, PD-L1)||MIH3||FC|
|Brilliant Violet 421™ anti-human CD274 (B7-H1, PD-L1)||MIH3||FC|
|PE anti-human CD274 (B7-H1, PD-L1)||MIH3||FC|
|FITC anti-human CD274 (B7-H1, PD-L1)||MIH3||FC|
|APC anti-human CD274 (B7-H1, PD-L1)||MIH3||FC|
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