FITC anti-human CD19 Antibody

Pricing & Availability
Clone
HIB19 (See other available formats)
Workshop
V CD19.11
Other Names
B4
Isotype
Mouse IgG1, κ
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Product Citations
publications
HIB19_FITC_061807
Human peripheral blood lymphocytes stained with HIB19 FITC
  • HIB19_FITC_061807
    Human peripheral blood lymphocytes stained with HIB19 FITC
See FITC spectral data
Cat # Size Price Quantity Avail. Save
302205 25 tests 36€
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302256 100 µg 72€
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302206 100 tests 80€
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Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Chimpanzee, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
µg sizes: 0.5 mg/ml
test sizes: lot-specific (please contact technical support for concentration and total µg amount)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

Application References
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Hansen IS, et al. 2018. Nat Commun. 9:863. PubMed
  2. Toepfner N et al. 2018. eLife. 7 pii: e29213. PubMed
  3. Kuri-Magaña H, et al. 2018. Front Immunol. 9:2679. PubMed
  4. Janela B, et al. 2019. Immunity. 50:1069. PubMed
  5. Zegarra‐Ruiz DF et al. 2018. Cell host & microbe. 25(1):113-127 . PubMed
  6. Beyer AI, et al. 2017. Stem Cells Dev. 26:102. PubMed
  7. Rusconi M, et al. 2017. Sci Rep. 5.745138889. PubMed
  8. Motwani MP, et al. 2018. JCI Insight. 3:e94463. PubMed
  9. Hazenberg MD, et al. 2019. Blood Adv. 2.659722222. PubMed
  10. Lefley D, et al. 2019. Breast Cancer Res. 0.965277778. PubMed
  11. Foote JR, et al. 2019. Front Immunol. 10:188. PubMed
  12. Łyszkiewicz M, et al. 2020. Nat Commun. 11:1031. PubMed
  13. Aravena O, et al. 2017. Arthritis Res Ther. 19:8. PubMed
  14. Hagan T, et al. 2020. Cell. 178(6):1313-1328.e13.. PubMed
  15. Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed
  16. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  17. Wang X, et al. 2010. Haematologica. 95:884. PubMed
  18. Lepore M, et al. 2014. J Exp Med. 211:1363. PubMed
  19. Jama B, Morris G 2014. J Vis Exp. 93: 52257. PubMed
  20. Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
  21. Muto M, et al. 2016. J Am Soc Nephrol. 10.1681/ASN.2016050496. PubMed
RRID
AB_314235 (BioLegend Cat. No. 302205)
AB_2564143 (BioLegend Cat. No. 302256)
AB_314236 (BioLegend Cat. No. 302206)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 10-03-2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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