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FITC anti-6-His Epitope Tag Antibody (Previously Covance catalog# FITC-156L)

Pricing & Availability
Clone
6-His (See other available formats)
Regulatory Status
RUO
Other Names
polyhistidine-tag, hexa histidine-tag, 6xHis-tag, His6 tag, His-tag(TM)
Previously
Covance Catalog# FITC-156L
Isotype
Mouse IgG1, κ
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Product Citations
publications
6-His_FITC_6-His_Epitope_Tag_062121.png
ICC staining of FITC anti-6-His Epitope Tag antibody (clone 6-His) on HeLa cells transfected with Posi-Tag plasmid. The cells were fixed with 4% PFA, and permeabilized with a buffer containing 0.5% Triton X-100, and blocked with 5% fetal bovine serum in PBS. The cells were then incubated with 10 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective.
  • 6-His_FITC_6-His_Epitope_Tag_062121.png
    ICC staining of FITC anti-6-His Epitope Tag antibody (clone 6-His) on HeLa cells transfected with Posi-Tag plasmid. The cells were fixed with 4% PFA, and permeabilized with a buffer containing 0.5% Triton X-100, and blocked with 5% fetal bovine serum in PBS. The cells were then incubated with 10 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective.
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906105 100 µL 336€
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Description

His tags are commonly used as a method of tagging proteins for detection or purification. This antibody is raised again a polyhistidine tag (6-His or hexa His-tag).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal + 50% Glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
Store between 2°C and 8°C.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The sequence of the protein flanking the poly His tag does not influence antibody binding. This antibody was subsequently purified via a protein-G column.

Application References
  1. Deinert K, et al. 2001. J. Biol. Chem. 276:6000. (WB)
  2. Kojima H, et al. 2001. J. Biol. Chem. 276:19238. (IF) PubMed
  3. Lapinski PE, et al. 2000. J. Biol. Chem. 275:6831. (WB)
  4. Bartsch D, et al. 1998. Cell. 95:211. (IF)
Product Citations
  1. Kojima H, et al. 2001. J Biol Chem. 276:19238-19243. PubMed
RRID
AB_2565059 (BioLegend Cat. No. 906105)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about 6-His on UniProt.org
Go To Top Version: 6    Revision Date: 03-12-2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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