Epitope Tag Small Motif Antibody Sampler Kit

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A_SmallMotifSampler_DYKDDDDK_WB_072717
50ng recombinant human IFN-γ protein (lane 1, negative control) and Posi-Tag Epitope Tag Protein (lane 2) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL Purified anti-DYKDDDDK Tag (clone L5) Antibody. Proteins were visualized using an HRP goat anti-mouse IgG (clone Poly4053) secondary antibody and chemiluminescence detection. Lane M: MW ladder. Lane M*: long exposure MW ladder.
  • A_SmallMotifSampler_DYKDDDDK_WB_072717
    50ng recombinant human IFN-γ protein (lane 1, negative control) and Posi-Tag Epitope Tag Protein (lane 2) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL Purified anti-DYKDDDDK Tag (clone L5) Antibody. Proteins were visualized using an HRP goat anti-mouse IgG (clone Poly4053) secondary antibody and chemiluminescence detection. Lane M: MW ladder. Lane M*: long exposure MW ladder.
  • B_SmallMotifSampler_His-Tag_WB_072717
    50ng recombinant human IFN-γ protein (lane 1, negative control) and Posi-Tag Epitope Tag Protein (lane 2) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 0.2 µg/mL Purified anti-His Tag (clone J099B12) Antibody. Proteins were visualized using an HRP goat anti-mouse IgG (clone Poly4053) secondary antibody and chemiluminescence detection. Lane M: MW ladder.
  • C_SmallMotifSampler_HA11_WB_072717
    15µg each of total protein from CHO cells (lane 3), and CHO transfected with HA tagged protein (lane 4) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL Purified anti-HA.11 Epitope Tag (clone 16B12) Antibody. Proteins were visualized using an HRP goat anti-mouse IgG (clone Poly4053) secondary antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) Antibody was used as a loading control. Lane M: MW ladder. Lane M*: long exposure MW ladder.
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Description

The Epitope Tag (Small Motif) Antibody Sampler Kit provides a convenient resource to detect small Epitope Tag fused protein by Western Blot. The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins. The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106. Anti-His tag antibody was raised against linear 8XHis tag. It reacts with His tag protein, either C, N, or internal His6 and up repeats. The 9E10 monoclonal antibody recognizes human myc and the 10 amino acid epitope tag of human c-myc. The V5 tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. For additional antigen details, please refer to individual product datasheets.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
Specificity Clone Size Reactivity Isotype MW (kD)
Anti-DYKDDDDK L5 25 µg DYKDDDDK tag epitope Rat IgG2a, κ N/A
Anti-c-Myc 9E10 25 µg EQKLISEEDL tag epitope Mouse IgG1 N/A
Anti-HA.11 16B12 25 µg YPYDVPDYA tag epitope Mouse IgG1, κ N/A
Anti-V5 Tag 7/4 25 µg GKPIPNPLLGLDST tag epitope Mouse IgG N/A
Anti-His Tag J099B12 25 µg 6x His tag epitope Mouse IgG1, κ N/A

* For detailed information about each specificity, please follow the provided links. 

Product Details

Formulation
Please refer to individual product datasheets for details.
Preparation
All antibodies in this kit were purified by affinity chromatography.
Concentration
All antibodies in the kit are at 0.5mg/ml
Storage & Handling
Upon receipt, store undiluted at 2-8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of antibodies in this kit is quality control tested by Western Blotting. For Western blotting, the suggested uses of these reagents are as follows:

Anti-DYKDDDDK: 0.1-1 µg/ml
Anti-Myc: 0.1-0.5 µg/ml
Anti-HA.11: 0.1-1 µg/ml
Anti-V5 tag: 0.1-0.5 µg/ml
Anti-His tag: 0.1-0.2 µg/ml

It is recommended that the reagent be titrated for optimal performance for each application.

Application References
  1. Sasaki M, et al. 2011. J. Biol Chem. 286:39370. PubMed (Anti-DYKDDDDK)
  2. Sonder SU, et al. 2012. J Immunol. 188:5906. PubMed (Anti-DYKDDDDK)
  3. Toyo-Oak K, et al. 2014. J Neurosci. 34:12168. PubMed (Anti-DYKDDDDK)
  4. Das A, et al. 2016. J. Biol. Chem.. 291: 6096 - 6110. PubMed (Anti-DYKDDDDK)
  5. Kondo, S., et al. 2011. J. Virol. 85:11255. PubMed (Anti-Myc)
  6. Bourgeois-Daigneault MC, et al. 2012. J Immunol. 188:4959. PubMed (Anti-Myc)
  7. Robu M, et al. 2013. PNAS. 110:1658. PubMed (Anti-Myc)
  8. Pecot MY, Malhotra V. 2004. Cell. 116:99. (Anti-HA.11)
  9. Görtz D, et al. 2015. Scientific Reports. 5: 14685. PubMed (Anti-HA.11)
  10. Thoms M, et al. 2016. Nucleic Acids Res. 44: 926 - 939. PubMed (Anti-HA.11)
  11. Burritt JB, et al. 1995. J. Biol. Chem. 270:16974. (Anti-His tag)
  12. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429. (Anti-His tag)
  13. Keshwani M, et al. 2015. Biochem. J.. 472: 329 - 338. PubMed (Anti-His tag)

Antigen Details

Biology Area
Cell Biology
Gene ID
4609 View all products for this Gene ID

Related FAQs

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Go To Top Version: 1    Revision Date: 07-26-2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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