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DyLight™ 649 Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
SAv-DyLight™ 649
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Product Citations
publications
DyLight649_Streptavidin_Antibody_042123
Human peripheral blood lymphocytes were stained with (DyLight 649) streptavidin alone (open histogram) or Biotin anti-human CD3 (clone UCHT1) followed by (DyLight 649) Streptavidin (filled histogram).
  • DyLight649_Streptavidin_Antibody_042123
    Human peripheral blood lymphocytes were stained with (DyLight 649) streptavidin alone (open histogram) or Biotin anti-human CD3 (clone UCHT1) followed by (DyLight 649) Streptavidin (filled histogram).
See DyLight® 649 spectral data
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405224 100 µg 95€
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Description

Streptavidin binds to biotin with high affinity. DyLight™ 649-conjugated Streptavidin is useful for detecting biotinylated antibodies. The excitation of DyLight™ 649 by 654 nm laser light induces a fluorescence emission maximum of 670 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
Streptavidin is conjugated with DyLight™ 649 under optimal conditions.
Concentration
0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC, IHC-F, IHC-P
Recommended Usage

This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the DyLight™ 649 conjugation. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Streptavidin-DyLight™ 649 is useful as a second step reagent for indirect immunofluorescent staining when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-DyLight™ 649 is 60 kD and Streptavidin alone is 52 kD.

Application References
  1. Van VQ, et al. 2012. PLoS One. 7:e41972. PubMed
  2. Zenker S, et al. 2014. J. Immunol. 192:2830. PubMed
Product Citations
  1. Van V, et al. 2012. PLoS One. 7:e41972. PubMed
  2. Zenker S, et al. 2014. J Immunol. 192:2830. PubMed
  3. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  4. Matsumoto S, et al. 2015. J Biol Chem. 290: 20071-20085. PubMed
  5. Ermert D, et al. 2016. PLoS Pathog. 11: 1005043. PubMed
  6. Carestia A, et al. 2019. Cell Rep. 28:896. PubMed
  7. Nowak W, et al. 2020. EBioMedicine. 50:290-305.. PubMed

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Go To Top Version: 7    Revision Date: 04-21-2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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