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Direct-Blot™ HRP anti-Granzyme B Antibody

Pricing & Availability
Clone
M3304B06 (See other available formats)
Regulatory Status
RUO
Other Names
Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase, CSPB, CTLA-1, CGL-1, CGL1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
M3304B06_DBHRP_GranzymeB_Antibody_051017
Total cell lysate from HeLa (lane 1, negative control) and PBMC (lane 2) (15 µg/lane) were resolved by electrophoresis (4-12% Bis-Tris), transferred to nitrocellulose, and probed with 1:250 purified anti-Granzyme B (clone M3304B06) and 1:1000, 1:2500 and 1:10000 Direct-Blot™ HRP anti- Granzyme B Antibody(upper) or loading control GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP for the purified antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for GAPDH. Lane M is the molecular weight ladder.
  • M3304B06_DBHRP_GranzymeB_Antibody_051017
    Total cell lysate from HeLa (lane 1, negative control) and PBMC (lane 2) (15 µg/lane) were resolved by electrophoresis (4-12% Bis-Tris), transferred to nitrocellulose, and probed with 1:250 purified anti-Granzyme B (clone M3304B06) and 1:1000, 1:2500 and 1:10000 Direct-Blot™ HRP anti- Granzyme B Antibody(upper) or loading control GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP for the purified antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for GAPDH. Lane M is the molecular weight ladder.
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674603 100 µL 272€
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Description

Granzyme B is a serine protease that is mainly produced by cytotoxic T cells (CTL) and NK cells. It plays important roles in cytotoxic lymphocyte-mediated apoptosis, chronic inflammation, and impaired wound healing. Granzyme B is packaged in cytoplasmic granules that are exocytosed towards a bound target cell, and subsequently activates multiple protein substrates to induce apoptosis. Perforin facilitates the transfer of Granzyme B into the target cell. Most circulating CD56+ CD8- NK cells and approximately half of circulating CD8+ T cells coexpress both Granzyme A and B. The activation of CD8+ and CD4+ T lymphocytes induces substantial expression of Granzyme B, but not Granzyme A. Recently, Granzyme B has been found to be produced by other cell types such as CD34+ hematopoietic progenitor cells, keratinocytes, basophils, mast cells, plasmacytoid dendritic cells, B cells, and smooth muscle cells. Elevated levels of Granzyme B are also found in some kinds of autoimmune diseases, type 1 diabetes, and cardiovascular diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Granzyme B produced in the 293E cell line.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1,000-1:10.000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

This antibody can be used for at least 20 western blots.

RRID
AB_2687057 (BioLegend Cat. No. 674603)

Antigen Details

Structure
247 amino acids with a molecular weight of approximately 28 kD
Distribution

Cytotoxic T cells, NK cells, and neutrophils.

Function
Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA / IL-2 and CD4+ T lymphocytes with anti-CD3/CD28 or anti-CD3/CD46.
Interaction
Targets of CTL and NK cells.
Cell Type
B cells, Dendritic cells, Neutrophils, NK cells, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Neuroscience, Signal Transduction
Molecular Family
Enzymes and Regulators, Proteases
Antigen References

1. Edwards KM, et al. 1999. J. Biol. Chem. 274:30468.
2. Grossman WJ, et al. 2004. Blood 104:2840.
3. Heusel JW, et al. 1994. Cell 76:977.
4. Schmid J and Weissmann C. 1987. J. Immunol. 139:250.
5. Trapani JA, et al. 1988. Proc. Natl. Acad. Sci. USA 85:6924.
6. Hiebert PR and Granville DJ. 2012. Trends Mol. Med. 18:732.
7. Saito Y, et al. 2011. J. Cardiol. 57:141.
8. Ewen CL, et al. 2012. Cell Death Differ. 1:28.

Gene ID
3002 View all products for this Gene ID
UniProt
View information about Granzyme B on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06-13-2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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  • Purified anti-Granzyme B

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