- Other Names
- (CEQKLISEEDL), Myc Proto-Oncogene Protein, Proto-Oncogene C-Myc, Transcription Factor P64, Class E Basic Helix-Loop-Helix Protein 39, Avian Myelocytomatosis Viral Oncogene Homolog, V-Myc, Myc-Related Translation/Localization
Covance Catalog# PEP-150P
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The c-myc tag is often used as a protein modification in order to simplify the labeling and detection of proteins. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to targeted proteins and allow for specific detection in western blotting, immunoprecipitation, and immunostaining techniques.Product Details
- >90% pure by HPLC
- Storage & Handling
- Store lyophilized or at -20°C in solution
- Recommended Usage
Soluble in PBS. The optimal working dilution should be determined for each specific assay condition.
• Elution of epitope tag fusion proteins from immunoaffinity columns: 400 ug/mL
• Competitive inhibition of antibody in western or immunostaining: 10-fold molar excess over optimized (low) antibody
- Application Notes
This synthetic peptide (H2N-CEQKLISEEDL-COOH) corresponds to the epitope detected by the 9E10 monoclonal antibody.
This peptide can be used in competition experiments, or to elute bound c-myc fusion proteins from the 9E10 affinity matrix.
- Application References
- Enquist-Newman M, et al. 2001. Mol. Biol. Cell. 12: 2601.
- Ferrando A, et al. 2001. Nucleic Acids Res. 29:3685.