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Brilliant Violet 785™ anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Regulatory Status
RUO
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
BM8_BV785_F480_Antibody_FC_061614.jpg
Thioglycolate-elicited BALB/c mouse peritoneal macrophages were stained with F4/80 (clone BM8) Brilliant Violet 785™ (filled histogram) or rat IgG2a, κ Brilliant Violet 785™ isotype control (open histogram).
  • BM8_BV785_F480_Antibody_FC_061614.jpg
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages were stained with F4/80 (clone BM8) Brilliant Violet 785™ (filled histogram) or rat IgG2a, κ Brilliant Violet 785™ isotype control (open histogram).
Compare all formats See Brilliant Violet 785™ spectral data
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123141 50 µg 220€
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Description

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.

Application References
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Haratani K, et al. 2019. J Clin Invest. 130:374. PubMed
  2. Scott CL et al. 2018. Immunity. 49(2):312-325 . PubMed
  3. Sepe JJ, et al. 2022. JACC Basic Transl Sci. 7:915. PubMed
  4. Kumaraswami K, et al. 2020. Int J Mol Sci. 21:00. PubMed
  5. Rao E, et al. 2021. Sci Immunol. 6:. PubMed
  6. Somerville TD, et al. 2020. eLife. 9:e53381.. PubMed
  7. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
  8. Simic M, et al. 2020. Cell Reports. 32(6):108004. PubMed
  9. Sodji QH, et al. 2022. Cancer Res Commun. 2:725. PubMed
  10. Bonnardel J, et al. 2019. Immunity. 51:638. PubMed
  11. Goossens P, et al. 2019. Cell Metab. 29:1376. PubMed
  12. Sharma M, et al. 2020. Circ Res. 127:335. PubMed
  13. Zeng W, et al. 2021. STAR Protocols. 2(1):100361. PubMed
  14. de Reuver R, et al. 2021. Cell Reports. 36(6):109500. PubMed
  15. Jorapur A, et al. 2022. PLoS Pathog. 18:e1010200. PubMed
  16. Germundson DL, et al. 2022. Front Allergy. 3:870513. PubMed
  17. Guilliams M, et al. 2022. Cell. 185:379. PubMed
  18. Gentek R, et al. 2018. Immunity. 48:1160. PubMed
  19. Mossadegh–Keller N, et al. 2019. Bio Protoc. 9:5. PubMed
  20. Etzerodt A, et al. 2019. J Exp Med. 216:2394. PubMed
  21. Liu Y, et al. 2019. Nat Commun. 10:324. PubMed
  22. Riggs JB, et al. 2021. Front Microbiol. 12:656979. PubMed
  23. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  24. Dai X, et al. 2021. Molecular Cell. 81(11):2317-2331.e6. PubMed
  25. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  26. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  27. Bosteels C, et al. 2020. Immunity. 52(6):1039-1056.e9.. PubMed
  28. Hartwig T et al. 2017. Molecular cell. 65(4):730-742 . PubMed
  29. Sharma M, et al. 2019. Immunometabolism. 1. PubMed
  30. Shen H, et al. 2022. Nat Commun. 13:5013. PubMed
  31. Catrysse L, et al. 2021. Cell Rep. 36:109748. PubMed
  32. Phadke MS, et al. 2021. Cancer Immunol Res. 9:554. PubMed
  33. Mincham KT, et al. 2021. Front Immunol. 11:601494. PubMed
  34. Subramanian S, et al. 2022. Nat Immunol. 23:458. PubMed
  35. Etzerodt A, et al. 2020. J Exp Med. 217:00:00. PubMed
  36. Goddery EN, et al. 2021. Front Immunol. 12:726421. PubMed
RRID
AB_2563667 (BioLegend Cat. No. 123141)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06-27-2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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