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Brilliant Violet 650™ anti-human CD279 (PD-1) Antibody

Pricing & Availability
Clone
NAT105 (See other available formats)
Regulatory Status
RUO
Other Names
PD1, PDCD1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
NAT105_BV650_CD279_Antibody_1_051118
Human peripheral blood lymphocytes were stained with CD3 FITC and CD279 (PD-1, clone NAT105) Brilliant Violet 650™ (left), or Mouse IgG1, κ Brilliant Violet 650™ isotype control (right).
  • NAT105_BV650_CD279_Antibody_1_051118
    Human peripheral blood lymphocytes were stained with CD3 FITC and CD279 (PD-1, clone NAT105) Brilliant Violet 650™ (left), or Mouse IgG1, κ Brilliant Violet 650™ isotype control (right).
  • NAT105_BV650_CD279_Antibody_2_051118
    PHA-stimulated (3-day) human peripheral blood lymphocytes were stained with CD279 (PD-1, clone NAT105) Brilliant Violet 650™ (filled histogram) or Mouse IgG1, κ Brilliant Violet 650™ isotype control (open histogram).
Compare all formats See Brilliant Violet 650™ spectral data
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367429 25 tests 168€
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367430 100 tests 335€
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Description

Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune diseases. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC, CD273) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. The interaction with CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
YT cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunofluorescence staining.

We recommend using our Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB) (Cat. No. 929501).

Application References
  1. Roncador G, et al. 2007. Haematologica 92:1059.
  2. Nam-Cha SH, et al. 2008. Am. J. Surg. Pathol. 32:1252.
  3. Rodriguez-Pinilla SM, et al. 2009. Am. J. Surg. Pathol. 33:81.
Product Citations
  1. Jin S, et al. 2020. Cell Res. 30:950. PubMed
RRID
AB_2734413 (BioLegend Cat. No. 367429)
AB_2734414 (BioLegend Cat. No. 367430)

Antigen Details

Structure
55 kD type I transmembrane protein.
Distribution

Transiently expressed on CD4- and CD8- thymocytes, upregulated in thymocytes and splenic T and B lymphocytes, and is expressed on activated myeloid cells.

Function
Signaling, co-stimulation (co-inhibition), immunoglobulin superfamily.
Interaction
SHP-1 and SHP-2.
Ligand/Receptor
PD-L1 (CD274) and PD-L2 (CD273).
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Francisco LM, Sage PT, and Sharpe AH. 2010. Immunological Rev. 236:219.

Gene ID
5133 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05-11-2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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