- PC61 (See other available formats)
- Regulatory Status
- Other Names
- IL-2Rα, Ly-43, p55, Tac
- Rat IgG1, λ
- Ave. Rating
- Submit a Review
- Product Citations
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CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- IL-2-dependent cytolytic mouse T-cell clone B6.1
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4, in vivo depletion of CD25+CD4+ Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102040) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.
- Application References
- Lowenthal JW, et al. 1985. Nature 315:669. (IP, Block)
- Ceredig R, et al. 1985. Nature 314:98. (IP, IHC, Block)
- Lowenthal JW, et al. 1985. J. Immunol. 135:3988. (Block)
- Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
- Takahashi T, et al. 2000. J. Exp. Med. 192:303. (Deplete)
- Onizuka S, et al. 1999. Cancer Res. 59:3128. (Deplete)
- Lei TC, et al. 2005. Blood 105:4865. (Deplete)
- Pasare C, et al. 2004. Immunity 21:733. (Deplete)
- León-Ponte M, et al. 2007. Blood 109:3139.
- Cao OW, et al. 2007. Blood doi:10.1182/blood-2007-02-073304. (Deplete)
- Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719.
- Liu F, et al. 2011. Arch Toxicol. 85:1383. PubMed
- Anguela XM, et al. 2013. Diabetes. 62:551. PubMed
- Product Citations
AB_2562269 (BioLegend Cat. No. 102041)
AB_2562270 (BioLegend Cat. No. 102042)
- Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells
- IL-2 receptor
- Cell Type
- B cells, T cells, Thymocytes, Tregs
- Biology Area
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
- Taniguchi T, et al. 1993. Cell 73:5-8.
- Waldmann TA. 1991. J Biol Chem. 266:2681-4.
- Read S, et al. 2000. J Exp Med. 192:295-302.
- Lowenthal JW, et al. 1985. J Immunol. 135:3988-94.
- Gene ID
- 16184 View all products for this Gene ID
- View information about CD25 on UniProt.org
Related Pages & Pathways
Other FormatsView All CD25 Reagents Request Custom Conjugation
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PerCP anti-mouse CD25
Brilliant Violet 421™ anti-mouse CD25
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Brilliant Violet 650™ anti-mouse CD25
Ultra-LEAF™ Purified anti-mouse CD25
Brilliant Violet 510™ anti-mouse CD25
PE/Dazzle™ 594 anti-mouse CD25
Brilliant Violet 711™ anti-mouse CD25
Brilliant Violet 785™ anti-mouse CD25
Alexa Fluor® 594 anti-mouse CD25
APC/Fire™ 750 anti-mouse CD25
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TotalSeq™-B0097 anti-mouse CD25
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