Brilliant Violet 421™ anti-mouse CD71 Antibody

Pricing & Availability
Clone
RI7217 (See other available formats)
Regulatory Status
RUO
Other Names
Transferrin receptor, T9
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RI7217_BV421_CD71_Antibody_1_060117
C57BL/6 mouse bone marrow cells were stained with Ter-119 APC and CD71 (clone RI7217) Brilliant Violet 421™ (top) or rat IgG2a, κ (clone RTK2758) Brilliant Violet 421™ isotype control (bottom).
  • RI7217_BV421_CD71_Antibody_1_060117
    C57BL/6 mouse bone marrow cells were stained with Ter-119 APC and CD71 (clone RI7217) Brilliant Violet 421™ (top) or rat IgG2a, κ (clone RTK2758) Brilliant Violet 421™ isotype control (bottom).
  • RI7217_BV421_CD71_Antibody_2_060117
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Avail. Save
113813 125 µL 148€
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Description

CD71 is a 95 kD type II heterodimeric transmembrane glycoprotein that is also known as T9 and transferrin receptor. CD71 is expressed on proliferating cells, reticulocytes, and erythroid precursors. Its expression is very low on resting leukocytes. CD71 plays a role in the control of cellular proliferation by facilitating the uptake of iron via ferrotransferrin binding and the recycling of apotransferrin to the cell surface.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of cellular proliferation. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 113821 & 113822).

This clone may also be known as R17217 or R17 217.

Application References
  1. Trowbridge I, et al. 1982. J. Cell. Physiol. 112:403.
  2. Grisendi S, et al. 2005. Nature 437:147.
  3. van Rooy I, et al. 2010. J. Control Release 150:30. PubMed
  4. Willhelm BT, et al. 2011. Blood 117:27. PubMed
  5. Okasi Y, et al. 2012. Exp Hematol. 40:143PubMed
  6. Stojanov K, et al. 2012. Mol Pharm. 9:1620. PubMed
Product Citations
  1. Tanimura N et al. 2018. Developmental cell. 46(5):581-594 . PubMed
  2. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  3. Nahrendorf W, et al. 2021. eLife. 10:00. PubMed
RRID
AB_10899739 (BioLegend Cat. No. 113813)

Antigen Details

Structure
Transferrin receptor and is 95 kD.
Distribution

Most dividing cells, the brain endothelium, and cycling hematopoietic progenitors.

Function
Mediates iron uptake.
Ligand/Receptor
Transferrin.
Cell Type
Hematopoietic stem and progenitors
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Hentze MW, et al. 1996. P. Natl. Acad. Sci. USA 93:8175.
2. Trowbridge IS, et al. 1993. Annu. Rev. Cell Biol. 9:129.
3. Trowbridge I, et al. 1982. J. Cell Physiol. 112:403.
4. Kuhn LC, et al. 1984. Cell 37:95.

Gene ID
22042 View all products for this Gene ID
UniProt
View information about CD71 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 11-30-2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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