Biotin anti-mouse CD120a (TNF R Type I/p55) Antibody

Pricing & Availability
Clone
55R-170 (See other available formats)
Other Names
TNFR type I, TNFRI, TNFR60, p55, TNFRSF1A
Isotype
Armenian Hamster IgG
Ave. Rating
1 reviews
Product Citations
publications
55R-170_Biotin_083107
NIH/3T3 cell line stained with biotinylated 55R-170, followed by Sav-PE
  • 55R-170_Biotin_083107
    NIH/3T3 cell line stained with biotinylated 55R-170, followed by Sav-PE
Cat # Size Price Quantity Avail. Save
112903 50 µg 60€
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112904 500 µg 184€
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Description

CD120a is a 55 kD Type I transmembrane protein, also known as Tumor Necrosis Factor Receptor Type I (TNFRI) or p55. It is expressed on a variety of cells at low levels. Resting T cells and erythrocytes express very little to no CD120a. This receptor binds both TNF-α and LT-α (also known as TNF-β). In association with TRADD and RIP, the receptor crosslinking induced by TNF-α or LT-α trimers is critical for signal transduction, leading to apoptosis, NF-kB activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state. The 55R-170 antibody has been shown to block in vitro and in vivo receptor signaling initiated by ligand binding.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
E. coli-expressed extracellular domain of the mouse TNFRI protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
ELISA Detection - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Flow Cytometry: For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 112904) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).
ELISA Detection1: The biotinylated 55R-170 antibody is useful as a detection antibody when paired with the purified 55R-286 antibody (Cat. No. 113002) as the capture antibody.
Additional reported applications (for the relevant formats) include:immunoprecipitation1, and In vitro and In vivo receptor blocking1. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 112902).

Application References
  1. Sheehan, K. C., et al. 1995. J. Exp. Med. 181:607.
RRID
AB_313528 (BioLegend Cat. No. 112903)
AB_313529 (BioLegend Cat. No. 112904)

Antigen Details

Structure
TNFR superfamily, 55 kD
Distribution
Variety of cell types at low levels
Function
Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation
Ligand/Receptor
TNF-α, LT-α (TNF-β)
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Aggarwal, B. B., et al. 1985. Nature 318 665.
2. Chan, F. K.M., et al. 2000. Science 288:2351.
3. Loetscher, H., et al. 1990. Cell 61:351.
4. Rothe, J., et al. 1993. Nature 364:798.

Gene ID
21937 View all products for this Gene ID
UniProt
View information about CD120a on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11-30-2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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