Biotin anti-human CD63 Antibody

Pricing & Availability
H5C6 (See other available formats)
Regulatory Status
HCDM listed
Other Names
LIMP, LAMP-3, Melanoma-associated antigen (ME491), Pltgp40, gp55
Mouse IgG1, κ
Ave. Rating
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Product Citations
Thrombin-activated platelets were stained with biotinylated CD63 (clone H5C6, filled histogram) or mouse IgG1, κ (open histogram) isotype control, followed by Sav-PE.
  • H5C6_Biotin_CD63_Antibody_FC_110512
    Thrombin-activated platelets were stained with biotinylated CD63 (clone H5C6, filled histogram) or mouse IgG1, κ (open histogram) isotype control, followed by Sav-PE.
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353017 50 µg 72€
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353018 200 µg 212€
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CD63 is a 53 kD type III lysosomal glycoprotein also known as LIMP, LAMP-3, gp55, and melanoma-associated antigen (ME491). CD63 is a member of the tetraspan transmembrane superfamily (TM4SF) protein expressed on activated platelets, monocytes/macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle cells. CD63 may be involved in platelet activation and is thought to function as a transmembrane adaptor protein. CD63 has been shown to associate with CD9, CD81, VLA-3, and VLA-6.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Host Species
T cell line HPB-ALL
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1, immunofluorescence2, and immunoprecipitation1.

Application References
  1. Hildreth JE, et al. 1991. Blood 77:121. (IP, WB)
  2. Beatty WL, et al. 2006. J. Cell Sci. 119:350. (IF)
Product Citations
  1. Kolan S, et al. 2015. Biochem Biophys Res Commun. 460:645. PubMed
  2. Yildizhan Y, et al. 2021. J Extracell Vesicles. e12059:10. PubMed
  3. Suthar J, et al. 2020. Anal Chem. 92:4082. PubMed
  4. Theodoraki MN, et al. 2018. Clin Cancer Res. 24:896. PubMed
  5. Wang J, et al. 2020. Sci Adv. 6:eaax3223. PubMed
  6. Theodoraki MN, et al. 2020. Oncoimmunology. 9:1747732. PubMed
  7. Kim D, et al. 2016. Proc Natl Acad Sci U S A. 113: 170 - 175. PubMed
  8. Zheng W, et al. 2022. Nat Biomed Eng. 6:979. PubMed
  9. Turiello R, et al. 2022. J Immunother Cancer. 10:. PubMed
  10. Theodoraki MN, et al. 2021. Br J Cancer. 125:1677. PubMed
  11. Köksal AR, et al. 2022. J Hepatocell Carcinoma. 9:959. PubMed
  12. Priglinger E, et al. 2021. J Extracell Vesicles. 10:e12156. PubMed
  13. Hofmann L, et al. 2022. Front Med (Lausanne). 9:904295. PubMed
  14. Hofmann L, et al. 2020. Int J Mol Sci. 21:00. PubMed
  15. Mondal SK, et al. 2021. Methods Mol Biol. 2265:305. PubMed
AB_2561675 (BioLegend Cat. No. 353017)
AB_2561676 (BioLegend Cat. No. 353018)

Antigen Details

Tetraspan transmembrane superfamily (TM4SF), type III lysosomal glycoprotein, 53 kD

Activated platelets, monocytes, macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle

Platelet activation
CD9, CD81, VLA-3, and VLA-6
Cell Type
B cells, Endothelial cells, Fibroblasts, Macrophages, Monocytes, Osteoclasts, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Azorsa DO, et al. 1991. Blood 78:280.
  2. Kishimoto T, et al. Eds. 1997. Leukocyte Typing V1. Oxford University Press New York.
  3. Hildreth JE, et al. 1991. Blood 77:121.
  4. Anzai N, et al. 2002. Blood 99:4413.
Gene ID
967 View all products for this Gene ID
View information about CD63 on

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 03-27-2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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