Loading...

APC Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin-Allophycocyanin, SAv-APC
Ave. Rating
Submit a Review
Product Citations
publications
APC_Streptavidin_Antibody_042123
Human peripheral blood lymphocytes were stained with APC streptavidin alone (open histogram) or Biotin anti-human CD3 (clone UCHT1) followed by (APC) Streptavidin (filled histogram).
  • APC_Streptavidin_Antibody_042123
    Human peripheral blood lymphocytes were stained with APC streptavidin alone (open histogram) or Biotin anti-human CD3 (clone UCHT1) followed by (APC) Streptavidin (filled histogram).
See APC spectral data
Cat # Size Price Quantity Check Availability Save
405207 100 µg 96€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Streptavidin binds to biotin with high affinity. Streptavidin-APC is useful for detecting biotinylated antibodies. The excitation of APC by 600-635 nm laser light induces a fluorescence emission maximum of 660 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin-APC solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this Streptavidin-APC is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the APC conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.125 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Streptavidin-APC is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-APC is 320 kD and Streptavidin alone is 52 kD.

Application References
  1. Kamimura D, et al. 2006. J. Immunol. 177:306.
  2. Andoniou CE, et al. 2005. Nature Immunology 6:1011.
  3. Chou WC,et al.2006.Blood108:3005. PubMed
  4. Malagolini N, et al. 2009. Glycobiology. 19:172. PubMed
  5. Kroeger KM, et al. 2009. J. Leukocyte Biol. PubMed
  6. Imada M, et al. 2009. Int. Immunol. 21:1151. PubMed
  7. Yi Y, et al. 2010 J. Biol Chem. 285:21233. PubMed
  8. King IL, et al. 2010. J. Immunol. 185:6138. PubMed
  9. Yokoyama T, et al. 2011. Int Immunol. PubMed
  10. Rusert P, et al. 2011. J. Exp Med. 208:1419. PubMed
  11. Bollig N, et al. 2012. PNAS. 109:8664. PubMed
  12. Nguyen V, et al. 2012. J. Immunol. 189:1081. PubMed
  13. Jager NA, et al. 2012. J Nucl Med. 53:1229. PubMed
  14. Liberman AC, et al. 2012. Mol Immunol. 50:220. PubMed
  15. Ogawa H, et al. 2012. Biochem Biophys Res Commun. 420:114. PubMed
  16. Ludin A, et al.2012. Nat Immunol. 13:1072. PubMed
  17. Sugiura D, et al. 2012. PLoS One. 7:e44770. PubMed
  18. Tani-Ichi S, et al. 2012. PNAS. 110:612. PubMed
  19. Jin L, et al. 2013. PNAS. 110:3907. PubMed
  20. Ota T, et al. 2013. J. Immunol. 191:3179. PubMed
  21. Fu X, et al. 2013. PNAS. 110:17982. PubMed
  22. Ryan SO, et al. 2014. Glycobiology. 24:362. PubMed
  23. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  24. Pilling D, et al. 2014. PLoS One. 9:93730. PubMed
  25. Matthews JA, et al. 2014. PLoS One. 9:97707. PubMed
  26. Owen KA, et al. 2014. PLoS Pathog. 10:1004159. PubMed
  27. Duek A, et al. 2014. Blood. 123:3943. PubMed
  28. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  29. Skogberg G, et al. 2014. J Immunol. 193:2187. PubMed
  30. Lundberg P, et al. 2014. J Exp Med. PubMed
  31. Martin RK, et al. 2014. PLoS One. 9:110609. PubMed
  32. Rai S, et al. 2014. PLoS One. 9:109441. PubMed
  33. Santoyo-Ramos P, et al. 2014. PLoS One. 9:112580. PubMed
  34. Jager NA, et al. 2014. J Nucl Med. 55:1945. PubMed
  35. Kong S, et al. 2014. J Immunol. 193:5515. PubMed
  36. Cabrera-Perez J, et al. 2015. J Immunol. 194:1609. PubMed
  37. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
  38. Kim Yu, et al. 2015. PLoS One. 10:120294. PubMed
  39. Zhang L, et al. 2015. J Immunol. 194:5243. PubMed
Product Citations
  1. Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
  2. Perruzza L, et al. 2019. Sci Rep. 9:9315. PubMed
  3. Cohen–Khait R, et al. 2017. Structure. 25:1867. PubMed
  4. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  5. Suzuki M, et al. 2015. J Immunol. 195: 1273-1281. PubMed
  6. Preece R, et al. 2020. Gene Ther. 451:27. PubMed
  7. Malagolini N, et al. 2009. Glycobiology. 0.911111111. PubMed
  8. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  9. Schneider C, et al. 2018. Cell. 174:271. PubMed
  10. Proietti M, et al. 2019. Nat Commun. 10:250. PubMed
  11. Tarban N, et al. 2022. Cells. 11:. PubMed
  12. Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
  13. Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed
  14. Georgiadis C, et al. 2021. Leukemia. . PubMed
  15. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  16. Viborg N, et al. 2019. Oncoimmunology. 8:e1663107. PubMed
  17. Pazina T, et al. 2019. Cancer Immunol Res. 7:1633. PubMed
  18. Temming AR, et al. 2021. Front Immunol. 12:594773. PubMed
  19. Blake SJ, et al. 2021. Cell Rep Med. 2:100464. PubMed
  20. Sarkar R, et al. 2021. Bio Protoc. 11:e4255. PubMed
  21. Hou S, et al. 2021. Sci Immunol. 6:eabg9433. PubMed
  22. Ota T, et al. 2013. J Immunol. 191:3179. PubMed
  23. Santoyo-Ramos P, et al. 2014. PLoS One. 9:112580. PubMed
  24. Kimura T, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 317:L271. PubMed
  25. Biffi G, et al. 2018. Cancer Discov. 2:282. PubMed
  26. Lebert-Ghali C, et al. 2017. Exp Hematol. 10.1016/j.exphem.2017.03.003. PubMed
  27. da Mota JB, et al. 2020. J Leukoc Biol. 108:1239. PubMed
  28. Niewolik D, et al. 2022. Nucleic Acids Res. 50:2096. PubMed
  29. Io S, et al. 2021. STAR Protoc. 2:100921. PubMed
  30. Yamanaka S, et al. 2021. STAR Protocols. 2(1):100314. PubMed
  31. J L, et al. 2016. Sci Rep. 6:30904. PubMed
  32. Gaudreault V, et al. 2015. PLoS One. 10: e0140805. PubMed
  33. Jager N, et al. 2014. J Nucl Med. 55:1945. PubMed
  34. Ludin A, et al. 2012. Nat Immunol. 13:1072. PubMed
  35. Jager N, et al. 2012. J Nucl Med. 53:1222. PubMed
  36. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  37. Catapano M, et al. 2020. J Invest Dermatol. 140:816. PubMed
  38. Rusert P, et al. 2011. J Exp Med. 208:1419. PubMed
  39. Mathews J, et al. 2014. PLoS One. 9:97707. PubMed
  40. Kim Y, et al. 2015. PLoS One. 10:120294. PubMed
  41. Jin L, et al. 2016. Stem Cell Res. 16:40-53. PubMed
  42. Palm A, et al. 2016. Sci Rep. 10.1038/srep20531. PubMed
  43. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  44. Liu C, et al. 2022. Cell Host Microbe. 30:53. PubMed
  45. She L, et al. 2021. JCI Insight. 6:e143509. PubMed
  46. Cao Y, et al. 2022. Nature. :. PubMed
  47. Sato Y, et al. 2021. J Clin Invest. Online ahead of print. PubMed
  48. Toulmin SA, et al. 2021. Nat Commun. 12:3993. PubMed
  49. Barcala Tabarrozzi A, et al. 2016. Sci Rep. 6:36646. PubMed
  50. Kong S, et al. 2014. J Immunol. 193:5515. PubMed
  51. Yokoyama T, et al. 2011. J Immunol. 208:885. PubMed
  52. Gui J, et al. 2020. Nat Cancer. 1:603. PubMed
  53. Duek A, et al. 2014. Blood. 123:3943. PubMed
  54. Bollig N, et al. 2012. Proc Natl Acad Sci U S A. 109:8664. PubMed
  55. Yi Y, et al. 2010. J Biol Chem. 285:21233. PubMed
  56. Chou W, et al. 2006. Blood. 108:3005. PubMed
  57. Tashita C, et al. 2020. World J Gastroenterol. 26:918. PubMed
  58. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  59. Cao Y, et al. 2022. Nature. 602:657. PubMed
  60. Kakizaki M, et al. 2021. Cell Death Dis. 12:1010. PubMed
  61. Calés C, et al. 2016. Mol Cell Biol. 36: 900 - 912. PubMed
  62. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
  63. Hansen UK, et al. 2020. Front Immunol. 11:373. PubMed
  64. Wang L, et al. 2022. Nat Commun. 13:4709. PubMed
  65. Kunitomi A, et al. 2022. Cell Rep Methods. 2:100317. PubMed
  66. Tani-ichi S, et al. 2012. Proc Natl Acad Sci U S A. 110:612. PubMed
  67. Ryan S, et al. 2014. Glycobiology. 24:262. PubMed
  68. Han X, et al. 2017. Mol Ther. 25(1):274-284. PubMed
  69. Hao Y, et al. 2020. J Biol Chem. . PubMed
  70. Parikh BA, et al. 2020. Cell Rep. 32:107969. PubMed
  71. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
  72. Mizoguchi I, et al. 2020. J Clin Invest. 130:6124. PubMed
  73. Locke F, et al. 2017. Mol Ther. 25(1):285-295. PubMed
  74. Jennings R, et al. 2016. Int Immunol. 28: 503 - 512. PubMed
  75. Morita N, et al. 2016. Innate Immunity. 10.1177/1753425916672312. PubMed
  76. Rai S, et al. 2014. PLoS One. 9:109441. PubMed
  77. Owen K, et al. 2014. PLoS Pathog. 10:1004159. PubMed
  78. Sugiura D, et al. 2012. PLoS One. 7:e44770. PubMed
  79. Audemard-Verger A, et al. 2017. J Immunol. 10.4049/jimmunol.1700430. PubMed
  80. Hermann BP, et al. 2018. Cell Rep. 25:1650. PubMed
  81. Van Gool F, et al. 2019. Immunity. 50:362. PubMed
  82. Friedrich SK, et al. 2021. Pathogens. 10: . PubMed
  83. Grioni M, et al. 2021. Blood Adv. 5:2817. PubMed
  84. Nguyen V, et al. 2012. J Immunol. 189: 1081. PubMed
  85. Jin L, et al. 2013. Proc Natl Acad Sci U S A. 110:3907. PubMed
  86. James KR, et al. 2020. Nat Immunol. 1.113194444. PubMed
  87. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  88. Guichard V, et al. 2017. Elife. 6:e27215. PubMed
  89. Pascual G, et al. 2021. Nature. 599:485. PubMed
  90. Saini SK, et al. 2021. Sci Immunol. 6:. PubMed
  91. Mainini F, et al. 2015. PLoS One. 10: 0145403. PubMed
  92. Zhang L, et al. 2015. J Immunol. 194:5423. PubMed
  93. Skogberg G, et al. 2014. J Immunol. 193:2187. PubMed
  94. Ogawa H, et al. 2012. Biochem Biophys Res Commun. 420:114. PubMed
  95. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  96. Jiang H, et al. 2011. PLoS One. 6:e27464. PubMed
  97. Collins DR, et al. 2021. Immunity. 54:2372. PubMed
  98. Larcombe-Young D, et al. 2022. STAR Protoc. 3:101414. PubMed
  99. Pilling D, Gomer R 2014. PLoS One. 9:93730. PubMed
  100. Dejnirattisai W, et al. 2021. Cell. 2183:184. PubMed
  101. King I, et al. 2010. J Immunol. 185:6138. PubMed
  102. Tuekprakhon A, et al. 2022. Cell. 185:2422. PubMed
  103. Kim SP, et al. 2022. Cancer Immunol Res. :OF1. PubMed
  104. Robertson TF, et al. 2021. J Cell Biol. 220:. PubMed
  105. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  106. Lundberg P, et al. 2014. J Exp Med. 211:2213. PubMed
  107. Budai Z, et al. 2021. Cells. 10:. PubMed
  108. Bainter W, et al. 2021. Sci Immunol. 6:eabf6723. PubMed
  109. Martin R, et al. 2014. PLoS One. 9:110609. PubMed
  110. Tian D, et al. 2019. Nat Commun. 10:4246. PubMed
  111. Gerdes P, et al. 2022. Nat Commun. 13:7470. PubMed
  112. Koide S, et al. 2022. iScience. 25:103603. PubMed
  113. Yewdell WT, et al. 2021. Cell Rep. 37:109961. PubMed
  114. Nguyen NT, et al. 2021. Nat Nanotechnol. 16:1424. PubMed
  115. Kosti P, et al. 2021. STAR Protocols. 2:100723. PubMed
  116. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
  117. Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed
  118. Mazor Y, et al. 2017. Sci Rep. 7:40098. PubMed
  119. Wan X, Thomas J, Unanue E 2016. J Exp Med. 213: 967 - 978. PubMed
  120. Liberman A, et al. 2012. Mol Immunol. 50:220. PubMed
  121. GR N, et al. 2012. PLoS One. 7:e39045. PubMed
  122. Pedersen NW, et al. 2019. Nat Commun. 10:837. PubMed
  123. Nagatake T, et al. 2019. Mucosal Immunol. 12:p1082. PubMed
  124. Yang C, et al. 2019. Nat Commun. 10:3931. PubMed
  125. Kroeger K, et al. 2009. J Leukoc Biol. 86:769. PubMed
  126. Zahradník J, et al. 2021. ACS Synth Biol. Online ahead of print. PubMed
  127. Chen Y, et al. 2021. Cancer Res Treat. Online ahead of print. PubMed
  128. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
  129. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  130. Tripathi S, et al. 2016. Proc Natl Acad Sci U S A. 113: 1555 - 1564. PubMed
  131. Hauser J, et al. 2016. Mol Immunol. 80:78-90. PubMed
  132. Wang W, et al. 2020. STAR Protoc. 1:100218. PubMed
  133. Fichtner ML, et al. 2022. Acta Neuropathol Commun. 10:154. PubMed
  134. Holm JS, et al. 2022. Nat Commun. 13:1935. PubMed
  135. Spanier J, et al. 2016. Nat Commun. 7:11804. PubMed
  136. Tagawa M, et al. 2016. PLoS One. 11: 0150030. PubMed
  137. Fu X, et al. 2013. Proc Natl Acad Sci U S A. 110:17892. PubMed

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 04-21-2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account