- NIM-R8 (See other available formats)
- Regulatory Status
- Other Names
- Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
|Cat #||Size||Price||Quantity Check Availability||Save|
CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.
- Excitation Laser
Red Laser (633 nm)
- Application Notes
From in-house testing, this clone does not block IM7. This clone has been used to induce dendrite formation in B cells.1
- Application References
- Santos-Argumedo L, et al. 1997. Immunology. 90:147 (FC, Agonist)
AB_2910319 (BioLegend Cat. No. 156003)
AB_2910319 (BioLegend Cat. No. 156004)
- Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells
- Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
- Hyaluronan, MIP-1β, fibronectin, collagen
- Cell Type
- Leukocytes, Epithelial cells, Endothelial cells, Mesenchymal cells, B cells
- Biology Area
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
- Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
- Haynes BF, et al. 1991. Cancer Cells 3:347.
- Goldstein LA, et al. 1989. Cell 56:1063.
- Mikecz K, et al. 1995. Nat. Med. 1:558.
- Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
- Liu T, et al. 2009. Biol. Direct 4:40.
- Gene ID
- 12505 View all products for this Gene ID
- View information about CD44 on UniProt.org
Related Pages & Pathways
Other FormatsView All CD44 Reagents Request Custom Conjugation
|Purified anti-mouse CD44||NIM-R8||FC|
|APC/Fire 750™ anti-mouse CD44||NIM-R8||FC|
|Pacific Blue™ anti-mouse CD44||NIM-R8||FC|
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.