APC/Fire™ 750 anti-human MERTK Antibody

Pricing & Availability
Clone
590H11G1E3 (See other available formats)
Other Names
C-Mer Proto-Oncogene Tyrosine Kinase, c-mer, Proto-Oncogene C-Mer, MER Receptor Tyrosine Kinase, STK Kinase, Receptor Tyrosine Kinase MerTK, Tyrosine-Protein Kinase Mer, MER, RP38, c-Eyk, Tyro12
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
590H11G1E3_APCFire_MERTK_Antibody_103017
Human peripheral blood monocytes were stimulated and cultured with M-CSF for seven days and stained with human MERTK (clone 590H11G1E3) APC/Fire™ 750 (filled histogram) or mouse IgG1, κ APC/Fire™ 750 isotype control (open histogram).
  • 590H11G1E3_APCFire_MERTK_Antibody_103017
    Human peripheral blood monocytes were stimulated and cultured with M-CSF for seven days and stained with human MERTK (clone 590H11G1E3) APC/Fire™ 750 (filled histogram) or mouse IgG1, κ APC/Fire™ 750 isotype control (open histogram).
See APC/Fire™ 750 spectral data
Cat # Size Price Quantity Avail. Save
367613 25 tests 108€
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367614 100 tests 244€
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Description

MERTK plays a role in the retinal pigment epithelium as a regulator of rod outer segments fragments phagocytosis. MERTK also plays a role in the inhibition of Toll-like receptor-mediated innate immune responses through the activation of STAT1. Upregulation of MERTK seems to also promote the survival of certain cancer cells, such as t(1;19)-positive acute lymphoblastic leukemias (ALL). MERTK also has a role in cellular migration, as MERTK KO macrophages demonstrate cytoskeletal disruptions that impacts its shape and directional migration. Melanoma cells express high levels of MERTK, which makes this molecule an attractive therapeutic target.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
MERTK extracellular domain/Fc fusion.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions. The solution is free of unconjugated APC/Fire™ 750 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

 

Excitation Laser
Red Laser (633 nm)
Application References
  1. Rogers AE, et al. 2012. Oncogene 31:4171.
RRID
AB_2721482 (BioLegend Cat. No. 367613)
AB_2721483 (BioLegend Cat. No. 367614)

Antigen Details

Structure
Transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type domains, and one tyrosine kinase domain.
Distribution

Macrophages, monocytes, and dendritic cells.

Function
TLR regulation, efferocytosis, phagocytosis regulation, cell survival, and macrophage migration.
Interaction
GRB2, PLCG2, and TNK2.
Ligand/Receptor
LGALS3, TUB, TULP1, and GAS6.
Cell Type
Dendritic cells, Macrophages, Monocytes
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
Molecular Family
Protein Kinases/Phosphatase, Toll Like Receptors
Antigen References

1. Schlegel J, et al. 2013. J. Clin. Invest. 123:2257.
2. Chen J, et al. 1997. Oncogene 14:2033.
3. Yefimova MG, et al. 2013. Autophagy 9:653.
4. Zhang W, et al. 2013. J. Med. Chem. 56:9693.
5. Lee YJ, et al. 2012. J. Leuko. Biol. 91:921.
6. Krause S, et al. 2015. Blood 125:820.
7. Tang Y, et al. 2015. PLoS One 10:e0117787.

Gene ID
10461 View all products for this Gene ID
UniProt
View information about MERTK on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 10-31-2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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