APC anti-human CD58 (LFA-3) Antibody

Pricing & Availability
Clone
TS2/9 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte function-associated antigen 3, LFA-3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
TS2slash9_APC_CD58_Antibody_021617
Human peripheral blood lymphocytes were stained with CD58 (clone TS2/9) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
  • TS2slash9_APC_CD58_Antibody_021617
    Human peripheral blood lymphocytes were stained with CD58 (clone TS2/9) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
See APC spectral data
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330917 25 tests 76,00€
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330918 100 tests 196,00€
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Description

CD58, also known as lymphocyte function-associated antigen 3 (LFA-3) is a 45-70 kD cell surface protein that is a member of the immunoglobulin superfamily. Alternative splicing of CD58 gives rise to transmembrane and glycosylphosphatidylinositol (GPI)-anchored forms on cell surface. CD58 is expressed on both hematopoietic and non-hematopoietic cells including B cells, T cells, monocytes, erythrocytes, endothelial cells, epithelial cells, and fibroblasts. High levels are observed on memory T cells and dendritic cells. CD58 expressed on antigen presenting cells and target cells enhances T cell recognition via the binding of it's cognate ligand, CD2, on the T cell surface.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human cytolytic T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications include: immunoprecipitation1, inhibition of cytolytic activity1, augment of IL-1 release by TE cells2

Application References
  1. Sanchez-Madrid F, et al. 1982. Proc. Nati Acad. Sci. USA. 79:7489
  2. Le PT, et al. 1990. J. Immunol. 144:4541
RRID
AB_2650885 (BioLegend Cat. No. 330917)
AB_2650886 (BioLegend Cat. No. 330918)

Antigen Details

Structure
Immunoglobulin superfamily member, 45-70 kD cell surface protein. Alternative splicing gives rise to transmembrane and glycosylphosphatidylinositol (GPI)-anchored form on cell surface.
Distribution

Expressed on both hematopoietic and non-hematopoietic cells including B cells, T cells, monocytes, erythrocytes, endothelial cells, epithelial cells, and fibroblasts. High levels are observed on memory T cells and dendritic cells.

Function
Mediates adhesion between various cell types (target cells and cytotoxic cells, APC and T cells, thymocytes and thymic epithelia).
Ligand/Receptor
CD2
Cell Type
B cells, Dendritic cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Springer TA, et al. 1987. Annu. Rev. Immunol. 5:223.
2. Dustin ML, et al. 1987. Nature 329:846.
3. Arulanandam AR, et al. 1994. J. Exp. Med. 180:1861.
4. Sanders ME, et al. 1988. J. Immunol. 140:1401.

Gene ID
965 View all products for this Gene ID
UniProt
View information about CD58 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 03-09-2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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