- Clone
- HTA125 (See other available formats)
- Workshop
- HCDM listed
- Other Names
- Toll-like Receptor 4, TLR4, TLR-4, CD284
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood monocytes were stained with CD284 (clone HTA125) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 312815 | 25 tests | 100€ | ||||
| 312816 | 100 tests | 228€ | ||||
Toll-like receptors are type I transmembrane signaling receptors. They are primordial pathogen-recognition proteins that function as sentinels for the innate immune system. TLR4, also known as CD284, is a 110 kD protein which is expressed on monocytes/macrophages, endothelial cells, and at low levels on B cells and granulocytes. In association with a secretory molecule, MD2, TLR4 has been recognized as critical for host recognition of bacterial LPS. HTA125 antibody is useful for flow cytometric analysis and is able to block LPS-induced cytokine production.
Product DetailsProduct Details
- Reactivity
- Human, Cross-Reactivity: Guinea Pig
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Ba/F3 cell line expressing human TLR4
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4, immunofluorescence microscopy6, Western blotting10, and in vitro blocking of LPS-induced cytokine production2,3,7,9. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 312806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 312804) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204). The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 312807). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 312814) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
- Application References
-
- Skimazu R, et al. 1999. J. Exp. Med. 189:1777.
- Wang R, et al. 2003. Hybrid Hybridomics 22:357. (Block)
- Wang JE, et al. 2001. Infect. Immun. 69:2402. (Block)
- Ishihara S, et al. 2004 J. Immunol. 173:1406. (IHC)
- Kawahara T, et al. 2001 Infect. Immun. 69:4382.
- Jiang Q, et al. 2000. J. Immunol. 165:3541. (IF)
- Sugawara S, et al. 2001. Infect. Immun. 69:4951. (Block)
- Chavakis E, et al. 2007. Circ. Res. 100:204. PubMed
- Bhattacharyya S, et al. 2007. Am. J. Physiol. Gastrointest Liver Physiol. doi:10.1152/ajpgi.00149. (Block) PubMed
- Baumgarten G, et al. 2001. J. Infectious. Dis. 183:1617.
- Product Citations
- RRID
-
AB_2562486 (BioLegend Cat. No. 312815)
AB_2562487 (BioLegend Cat. No. 312816)
Antigen Details
- Structure
- Type I transmembrane protein, Toll-like receptor family/IL-1R superfamily, 110 kD
- Distribution
-
Monocytes/macrophages, endothelial cells, B cells and granulocytes
- Function
- Activates the innate immune system against Gram-negative bacterial pathogens by recognition of LPS
- Ligand/Receptor
- Bacterial LPS
- Cell Type
- Monocytes, Macrophages, Endothelial cells, B cells, Granulocytes, Tregs
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Toll Like Receptors
- Antigen References
-
1. Skimazu R, et al. 1999. J. Exp. Med. 189:1777.
- Gene ID
- 7099 View all products for this Gene ID
- UniProt
- View information about CD284 on UniProt.org
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