- SK7; SK3; HI30;
- Regulatory Status
- Mouse IgG1, κ (all clones)
- Ave. Rating
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- Product Citations
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CD3: SK7 antibody reacts with a combinatorial epitope of CD3ε, a 20 kD chain of the CD3/T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells, and some thymocytes.
CD4: SK3 antibody reacts with CD4, a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages.
CD45: HI30 antibody reacts with CD45, a 180-240 kD single chain type I membrane glycoprotein which is also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets.
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- This reagent is a combination of FITC conjugated SK7, PE conjugated SK3, and PerCP conjugated HI30 at optimal concentration for three-color flow cytometric analysis.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood.
- Application Notes
This cocktail has been optimized to be used in a lyse-wash and lyse-no wash assay.
Single color controls are required to perform instrument compensation and are not included in the kit.
AB_2566176 (BioLegend Cat. No. 368302)
T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells, some thymocytes, and hematopoietic cells (not expressed in circulating erythrocytes or platelets).
- Cell Type
- Mesenchymal Stem Cells
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Antigen References
1. Kan EA, et al. 1983. J. Immunol. 131:536.
2. Wood GS, et al. 1985. Am. J. Pathol. 120:371.
3. van Dongen JJ, et al. 1988. Blood 71:603.
4. Center D, et al. 1996. Immunol. Today 17:476.
5. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.
6. Knapp W, et al. (1989) Leucocyte Typing IV. New York, NY: Oxford University Press.
7. Kishihara K, et al. 1993. Cell 74:143.
8. Esser M, et al. 2001. J. Virol. 75:6173.
- Gene ID
- 916 View all products for this Gene ID 920 View all products for this Gene ID 5788 View all products for this Gene ID
- View information about CD3/CD4/CD45 on UniProt.org
- How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?
PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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