Anti-HA Tag Antibody (Previously Covance catalog# PRB-101C)

Pricing & Availability
Clone
Poly9023 (See other available formats)
Regulatory Status
RUO
Other Names
HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Previously
Covance Catalog# PRB-101C
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly9023_Purified_HA-Tag_Antibody_WB_032615
Western blot analysis of HA-Tag antibody to Postitag control protein.
  • Poly9023_Purified_HA-Tag_Antibody_WB_032615
    Western blot analysis of HA-Tag antibody to Postitag control protein.
Cat # Size Price Quantity Check Availability Save
923501 200 µL 266,00€
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923502 500 µL 419,00€
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Description

Influenza Hemagglutinin (HA) is a viral membrane glycoprotein responsible for binding of the virus to the cells expressing sialic acid-containing surface receptors, such as erythrocytes and the cells in the upper respiratory tract. The hemagglutinin epitope, YPYDVPDYA, has been widely used as a tag in recombinant protein expression vectors.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Polyclonal antibody HA.11 was raised against the twelve amino acid sequence CYPYDVPDYASL.
Preparation
Serum
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application

WB, IP

Recommended Usage

The optimal working dilution should be determined for each specific assay condition.
WB: 1:1,000*
IP: 1:150

Application Notes

This antibody is effective in immunoblotting (WB) and immunoprecipitation (IP) of tagged proteins.

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

This antibody recognizes the same hemagglutinin flu epitope YPYDVPDYA as the monoclonal antibody, HA.11 clone 16B12. This polyclonal antibody facilitates epitope tag experiments in murine systems and is useful in experiments involving two proteins labeled with two different tags.


This product may contain other non-IgG subtypes.

Application References
  1. Barrott JJ, et al. 2011. PNAS. 108: 12752. (WB, IP)
  2. Pecot MY, Malhotra V. 2004. Cell. 116:99.
  3. Delmas C, et al. 2003. J Biol Chem. 278:12443. (IF)
  4. Caplan S, et al. 2001. J Cell Biol. 154:109. (IF, WB)
  5. Yeo C, Whitman M. 2001. Mol Cell. 7:949.
  6. Sander TL, et al. 2000. J Biol Chem. 275:12857. (WB)
  7. Bosshart H, et al. 1994. J Cell Biol. 126:1157. (IF, IP)
Product Citations
  1. Trautz B, et al. 2016. J Virol. 90(23):10915-10927. PubMed
  2. Zhu Z, et al. 2016. J Virol. 90(24):11106-11121. PubMed
  3. Villeneuve J, et al. 2017. Mol Biol Cell. 28(1):141-151. PubMed
  4. Ting Y, et al. 2017. J Biol Chem. 292(2):585-596. PubMed
  5. Li J, et al. 2021. Med (N Y). 0.152083333. PubMed
  6. Zeng J, et al. 2020. Cell Stem Cell. 27(4):618-632.e9. PubMed
RRID
AB_2565437 (BioLegend Cat. No. 923501)
AB_2565438 (BioLegend Cat. No. 923502)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about HA Tag on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 02-14-2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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