Anti-Alpha Smooth Muscle Actin Antibody (Previously Covance catalog# MMS-466S)

Pricing & Availability
1A4 (See other available formats)
Regulatory Status
HCDM listed
Other Names
Actin, aortic smooth muscle, alpha 2 actin, alpha-actin-2, alpha-cardiac actin, growth-inhibiting gene 46, cell growth-inhibiting gene 46 protein
Covance Catalog# MMS-466S
Mouse IgG2a
Ave. Rating
Submit a Review
Product Citations
Immunohistochemical staining of Smooth Muscle Actin (1A4) Monoclonal Antibody, on formalin fixed paraffin embedded human breast tissue at 1/50 dilution.
  • 1A4_Smooth-Muscle-Actin_Antibody_IHC_041515
    Immunohistochemical staining of Smooth Muscle Actin (1A4) Monoclonal Antibody, on formalin fixed paraffin embedded human breast tissue at 1/50 dilution.
Cat # Size Price Quantity Check Availability Save
904601 250 µL 282€
Check Availability

Need larger quantities of this item?
Request Bulk Quote
This product is scheduled for discontinuation. Please use the availability checker to determine current availability. Cat. Nos. 614851 & 614852 are available as an alternative.

Cytoplasmic actins, which belong to the microfilament system of cytoskeleton proteins, are some of the most conserved eukaryotic proteins being expressed in mammals and birds. The actin protein consists of six isoforms, varying in their amino acid sequence, but all having the same molecular mass of 42 kD. The isoforms show more than 90% overall sequence homology, but only 50-60% homology in their 18 N-terminal residues. The N-terminal region appears to be a major antigenic region. There are different α isoforms specific for muscle tissues, i.e. skeletal muscle α cardiac muscle α and smooth muscle α respectively. The β- and γ-actins may be present in muscle cells as well as most other cell types in the body, including non-muscle cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, Cow, Chicken
Antibody Type
Host Species
This antibody was raised against the N-terminal synthetic decapeptide of alpha-smooth muscle actin coupled to keyhole limpet haemocyanin (KLH).
Cell Culture Supernatant (dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3).
Tissue Culture Supernatant
Storage & Handling
Store between 2°C and 8°C.


Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
WB: 1:100*
IHC: 1:50-1:100**

Paraffin sections: This antibody can be used for labelling paraffin-embedded tissue sections fixed in formalin. Pre-treatment of tissues with heat-induced epitope retrieval is recommended. Optimal results are obtained with 10 mmol/L Tris buffer, 1 mmol/L EDTA, pH 9.0. Pre-treatment of tissues with proteinase-K was found destructive of the epitope. The tissue sections should not dry out during the treatment or during the following immunocytochemical staining procedure.
Frozen sections and cell preparations: The antibody can also be used for labelling acetone-fixed, frozen sections.

Application Notes

This antibody is effective in immunoblotting (WB), immunocytochemistry (ICC) and immunohistochemistry (IHC).

*Predicted MW = 42 kD

**Reccomend 20 minute heat-induced epitope retrieval in 10 mmol/L Tris buffer, 1 mmol/L EDTA, pH 9.0, and 30 minute incubation of primary antibody at room temperature.

Application References
  1. Brennan PA, et al. 2000. J Oral Pathol Med. 29:279.
  2. Rizeq MN, et al. 1994. Hum Pathol. 25:671.
  3. Schmitt-Graff A, et al. 1991. Am J Pathol. 138:1233.
  4. Roholl PJM, et al 1990. Hum Pathol. 21:1269.
  5. Alberts B, et al. 1989. Eds. Molecular biology of the cell. 2:613.
  6. Skalli O, et al. 1986. J Cell Biol. 103:2787.
Product Citations
  1. Nam MO, et al. 2018. Oncotarget. 9:6356. PubMed
  2. Eremichev R, et al. 2021. Front Cell Dev Biol. 616893:9. PubMed
  3. Mao C, et al. 2020. Sci Adv. 6:eaba4542. PubMed
  4. Chozinski TJ, et al. 2018. Sci Rep. 15:4005. PubMed
  5. Lin CY, et al. 2022. Int J Mol Sci. 23:. PubMed
AB_2565041 (BioLegend Cat. No. 904601)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Gene ID
59 View all products for this Gene ID
View information about Alpha Smooth Muscle Actin on

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 04-27-2023

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Anti-Alpha Smooth Muscle Actin

  • Purified anti-α Smooth Muscle Actin


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account