Alexa Fluor® 700 anti-human CD172a/b (SIRPα/β) Antibody

Pricing & Availability
Clone
SE5A5 (See other available formats)
Regulatory Status
RUO
Other Names
SIRPa, SIRPb, SIRPalpha/beta, BIT, SHPS1, MFR, P84, PTPNS1, CD172 antigen-like family member A, CD172 antigen-like family member B
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SE5A5_A700_CD172ab_Antibody_052217
Human peripheral blood granulocytes were stained with anti-human CD172a/b (SIRPα/β) Alexa Fluor® 700 (clone SE5A5, filled histogram) or mouse IgG1, κ Alexa Fluor® 700 isotype control (open histogram).
  • SE5A5_A700_CD172ab_Antibody_052217
    Human peripheral blood granulocytes were stained with anti-human CD172a/b (SIRPα/β) Alexa Fluor® 700 (clone SE5A5, filled histogram) or mouse IgG1, κ Alexa Fluor® 700 isotype control (open histogram).
See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Avail. Save
323815 25 tests 92,00€
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323816 100 tests 228,00€
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Description

CD172a, also known as signal-regulatory protein α (SIRPα), src homology 2 domain-containing phosphatase substrate-1 (SHPS1), PTPNS1, BIT, MFR, and P84, is a 75-110 kD transmembrane glycoprotein involved in receptor tyrosine kinase coupled signaling pathway. It belongs to the Ig superfamily and is primarily expressed on monocytes/macrophages, granulocytes, dendritic cells, and neurons. CD172a serves as a substrate of activated receptor tyrosine kinases (RTKs). The interaction of CD172a intracellular domain with SHP-1 and SHP-2 displays negative signaling in the regulation of leukocyte adhesion and transmigration, T cell activation, macrophage fusion, and phagocytosis. CD47 (IAP) is the extracellular ligand for CD172a. SIRPα was recently demonstrated to be a specifc marker for cardiomyocytes derived from human pluripotent stem cells2.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NIH-3T3/hu-SIRPα cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Clone SE5A5 recognizes a common epitope on SIRPa (90 kD) and SIRPß (50 kD)3. A high degree of homology has been found between SIRP family isoforms alpha and beta at the level of extracellular domains. Consequently, many anti SIRP antibody clones, such as SE5A5, have been reported to cross react with several SIRP isoforms1,4,5. It reacts with CD172a and has weak cross-reaction with CD172b. This antibody is able to block the binding of SIRPa (SIRPa1 and SIRPa2) to CD471,6.

Application References
  1. Seiffert M, et al. 1999. Blood 94:3633.
  2. Dubois NC, et al. 2011. Nat. Biotechnol. 29:1011.
  3. Barros MM, et al. 2009. Transfusion 49:154.
  4. Liu Y, et al. 2005. J. Biol. Chem. 280:36132.
  5. Barclay AN. 2009. Curr. Opin. Immunol. 21:47.
  6. Florian S, et al. 2005. J. Leukoc. Biol. 77:984.
Product Citations
  1. Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed
RRID
AB_2687274 (BioLegend Cat. No. 323815)
AB_2687275 (BioLegend Cat. No. 323816)

Antigen Details

Structure
75-110 kD glycoprotein , signal-regulatorey-protein family, Ig superfamily
Distribution

Monocytes/macrophages, granulocytes, dendritic cells, neurons, cardiomyocytes

Function
Negative regulation of leukocyte adhesion and transmigration, macrophage fusion, phagocytosis
Ligand/Receptor
CD47
Cell Type
Dendritic cells, Granulocytes, Macrophages, Monocytes, Neurons
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Seiffert M, et al. 1999. Blood 94:3633.
2. Seiffert M, et al. 2001. Blood 97:2741.
3. Timms JF, et al. 1998. Mol. Cell Biol. 18:3838.
4. Barclay AN and Brown MH. 2006. Nat. Rev. Immnuol. 6:457.

Gene ID
140885 View all products for this Gene ID
UniProt
View information about CD172a/b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 05-23-2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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