Alexa Fluor® 647 anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
G8.8 (See other available formats)
Regulatory Status
RUO
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2a, κ
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Product Citations
publications
G8dot8_A647_081908.jpg
TE-71 (mouse thymic epithelial stromal cell line) stained with G8.8 Alexa Fluor® 647
  • G8dot8_A647_081908.jpg
    TE-71 (mouse thymic epithelial stromal cell line) stained with G8.8 Alexa Fluor® 647
  • G8dot8_A647_CD326_Antibody_2_121018
    Dissected C57/B6 mouse small intestine was immersed in 4% paraformaldehyde (PFA) overnight followed by 30% sucrose immersion overnight and frozen in OCT. Frozen section was blocked with 5% FBS and 5% mouse serum for 30 minutes at room temperature. Then the tissue section was stained with 2.5 µg/mL of anti-mouse Tubulin Beta 3 (clone AA10) Alexa Fluor® 488 (green) and 2.5 µg/mL of anti-mouse CD326 (clone G8.8) Alexa Fluor® 647 (red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • G8dot8_A647_CD326_Ep-CAM_Antibody_3D_IHC_05112021.png
    Paraformaldehyde-fixed (4%), 500 μm-thick mouse thymus tissue section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse CD3 Antibody (clone 17A2) Alexa Fluor® 594 at 5 µg/mL (green), and anti-mouse CD326 (Ep-CAM) Antibody (clone G8.8) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
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See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
118211 25 µg 84€
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118212 100 µg 180€
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Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TE-71 thymic epithelial cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD326 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, 3D IHC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 µg/ml is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4.

Application References
  1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
  2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
  3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
  4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
  5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
  6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  7. Ishii K, et al. 2012. Development. 139:1734. PubMed
  8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
Product Citations
  1. McClendon J, et al. 2017. Am J Pathol. 10.1016/j.ajpath.2017.04.012. PubMed
  2. Zhang J, et al. 2018. Sci Rep. 8:2373. PubMed
  3. Campbell C et al. 2018. Immunity. 48(6):1245-1257 . PubMed
  4. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  5. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  6. Dravis C et al. 2018. Cancer cell. 34(3):466-482 . PubMed
  7. Giraddi RR et al. 2018. Cell reports. 24(6):1653-1666 . PubMed
  8. Biffi G, et al. 2018. Cancer Discov. 2:282. PubMed
  9. Riemondy KA, et al. 2019. JCI Insight. 5:. PubMed
  10. Joshi PA, et al. 2019. Nat Commun. 10:1760. PubMed
  11. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  12. Chung CY et al. 2019. Cell Rep. 29(2):495-510 . PubMed
  13. Burns KA, et al. 2018. Endocrinology. 159:103. PubMed
  14. Bernstock JD, et al. 2019. J Allergy Clin Immunol. 145:358. PubMed
  15. Miragaia RJ, et al. 2018. Sci Rep. 8:685. PubMed
  16. Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
  17. Shaffer M, et al. 2010. PLoS One. 5:e12404. PubMed
  18. Gaiser M, et al. 2012. Proc Natl Acad Sci U S A. 1.034027778. PubMed
  19. Molaro A, et al. 2014. Genes Dev. 28:1544. PubMed
  20. Bretz N, et al. 2014. Immunol Lett. 161:140. PubMed
  21. Blijswijk J, et al. 2015. J Immunol. 194:307. PubMed
  22. Davis F, et al. 2015. Proc Natl Acad Sci U S A. 112:5827. PubMed
  23. Wasik K, et al. 2015. Genes Dev. 29: 1403 - 1415. PubMed
  24. Hegab A, et al. 2015. Stem Cell Res. 15: 109-121. PubMed
  25. Giraddi R, et al. 2015. Nat Commun. 6: 8487. PubMed
  26. Heijden M, et al. 2016. Nat Commun. 7:10916. PubMed
  27. Cheng Q, et al. 2020. Nat Nanotechnol. 0.842361111. PubMed
  28. Tang R, et al. 2020. Elife. 9:00. PubMed
  29. Strunz M, et al. 2020. Nat Commun. 2.929861111. PubMed
  30. Madenspacher JH, et al. 2020. JCI Insight. 5:00. PubMed
  31. Gabitova-Cornell L, et al. 2020. Cancer Cell. 38(4):567-583.e11. PubMed
  32. Bilate AM, et al. 2020. Immunity. 53(5):1001-1014.e20. PubMed
  33. Liu Y, et al. 2020. Cell. 183(6):1665-1681.e18. PubMed
  34. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
RRID
AB_1134104 (BioLegend Cat. No. 118211)
AB_1134101 (BioLegend Cat. No. 118212)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function
Mediates calcium-independent homophilic cell-cell adhesion.
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 05-11-2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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