Alexa Fluor® 594 anti-human Granzyme K Antibody

Pricing & Availability
Clone
GM26E7 (See other available formats)
Other Names
Tryptase II, Granzyme-3, fragmentin-3, GZMK
Isotype
Mouse IgG1, κ
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Product Citations
publications
GM26E7_A594_GranzymeK_Antibody_ICC_022819
Human peripheral blood mononuclear cells (PBMCs) were separated from whole blood using a Ficol Gradient method. The PBMCs were washed and fixed with 2% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes and blocked with 0.5% FBS for 30 minutes. After blocking, the cells were incubated with Granzyme K (clone GM26E7) Alexa Fluor® 594 (red) and CD8 (clone HIT8a) Alexa Fluor® 488 (green) for 30 minutes at room temperature. The cells were spun onto a microscopy slide with Cytospin. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
  • GM26E7_A594_GranzymeK_Antibody_ICC_022819
    Human peripheral blood mononuclear cells (PBMCs) were separated from whole blood using a Ficol Gradient method. The PBMCs were washed and fixed with 2% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes and blocked with 0.5% FBS for 30 minutes. After blocking, the cells were incubated with Granzyme K (clone GM26E7) Alexa Fluor® 594 (red) and CD8 (clone HIT8a) Alexa Fluor® 488 (green) for 30 minutes at room temperature. The cells were spun onto a microscopy slide with Cytospin. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
370506 100 µg 236€
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Description

Granzyme K is a 29 kD serine protease found in cytoplasmic granules of cytotoxic lymphocytes and NK cells. Granzyme K is thought to induce cell death and lysis in response to non-self antigens on the cell surface by cleaving nucleosome assembly protein SET. Granzyme K is upregulated in several diseases associated with inflammation including arthritis, atherosclerosis, and asthma.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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RRID
AB_2629600 (BioLegend Cat. No. 370506)

Antigen Details

Structure
Serine Protease, approximately 29 kD.
Distribution

Activated CD8+ cytotoxic T-cells, natural killer (NK) cells, and CD8+ T-cells.

Function
Cytotoxicity.
Interaction
SET
Cell Type
NK cells, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
Molecular Family
Enzymes and Regulators, Proteases
Antigen References

1. Vrazo AC, et al. 2015. Blood 126.
2. Cooper DM, et al. 2011. PLoS. One 6.
3. Wensink AC, et al. 2015. J. Immunol. 194:491.
4. Zhao T, et al. 2007. Cell Death Differ. 14:489.

Gene ID
3003 View all products for this Gene ID
UniProt
View information about Granzyme K on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07-07-2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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