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Alexa Fluor® 488 anti-SSRP1 Antibody

Pricing & Availability
Clone
3E4 (See other available formats)
Regulatory Status
RUO
Other Names
Structure specific recognition protein 1 (SSRP1), Facilitates Chromatin Transcription 80 kD subunit, FACTp80, Recombination signal sequence recognition protein
Isotype
Mouse IgG1, κ
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Product Citations
publications
3E4_A488_SSRP1_Antibody_080719
HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 µg/ml anti-SSRP1 (clone 3E4) Alexa Fluor® 488 (green) in 5% FBS, overnight at 4°C, followed by Alexa Fluor® 647 Phalloidin (red) staining for 20 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
  • 3E4_A488_SSRP1_Antibody_080719
    HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 µg/ml anti-SSRP1 (clone 3E4) Alexa Fluor® 488 (green) in 5% FBS, overnight at 4°C, followed by Alexa Fluor® 647 Phalloidin (red) staining for 20 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
Compare all formats See Alexa Fluor® 488 spectral data
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609810 100 µg 287€
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Description

SSRP1 is a nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT140 complex is composed of the Spt16 and the SSRP1 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. This protein interacts with dsDNA. The SSRP1 protein has been shown to be modified by cisplatin and may protect DNA from repair and block DNA replication. The 3E4 monoclonal antibody recognizes human SSRP1 and has been shown to be useful for Western blotting.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant (partial)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Application References
  1. Tan B C-M, et al. 2006. EMBO doi:10.1038/sj.emboj.7601271
Product Citations
  1. Baljinnyam E, et al. 2017. Physiol Rep. 10.14814/phy2.13308. PubMed
RRID
AB_2566635 (BioLegend Cat. No. 609810)

Antigen Details

Structure
SSRP family, HMG box domain; 81 kD
Distribution

Nuclear

Function
Interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation, binds to dsDNA. Modified by cisplatin, may protect DNA from repair and block DNA replication
Interaction
Component of FACT ATP-dependent chromatin remodeling complex, Spt16/Cdc68 in FACT complex, H2A/H2B dimers
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, DNA Repair/Replication, Transcription Factors
Antigen References

1. LeRoy G, et al. 1998. Science 282:1836.
2. Orphanides G, et al. 1998. Cell 92:105.
3. Orphanides G, et al. 1999. Nature 400:284.

Gene ID
6749 View all products for this Gene ID
UniProt
View information about SSRP1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 08-07-2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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