- 500A2 (See other available formats)
- Regulatory Status
- Other Names
- CD3ε, T3, CD3
- Syrian Hamster IgG
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- Product Citations
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CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Syrian Hamster
- Mouse T cell receptor.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
IHC-F, 3D IHC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Application Notes
Clone 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.
- Application References
- Miescher GC, et al. 1989. Immunol. Lett. 23:113.
AB_2783123 (BioLegend Cat. No. 152321)
AB_2783124 (BioLegend Cat. No. 152322)
- Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD.
Thymocytes (differentiation dependent), mature T cells and NK-T cells.
- TCR signal transduction, T cell activation and antigen recognition.
- T-cell receptor (TCR).
- Peptide antigen/MHC-complex.
- Cell Type
- NKT cells, T cells, Thymocytes, Tregs
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Molecular Family
- CD Molecules, TCRs
- Antigen References
1. Barclay A, et al. 1997. The Leucocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell. 76:263.
- Gene ID
- 12501 View all products for this Gene ID
- View information about CD3epsilon on UniProt.org
Related Pages & Pathways
Other FormatsView All CD3ε Reagents Request Custom Conjugation
|APC anti-mouse CD3ε||500A2||FC|
|FITC anti-mouse CD3ε||500A2||FC|
|PerCP/Cyanine5.5 anti-mouse CD3ε||500A2||FC|
|PE/Cyanine7 anti-mouse CD3ε||500A2||FC|
|PE anti-mouse CD3ε||500A2||FC|
|APC/Fire™ 750 anti-mouse CD3ε||500A2||FC|
|Alexa Fluor® 700 anti-mouse CD3ε||500A2||FC|
|Purified anti-mouse CD3ε||500A2||FC,IHC-F,Block|
|Alexa Fluor® 594 anti-mouse CD3ε||500A2||IHC-F,3D IHC|
|Alexa Fluor® 647 anti-mouse CD3ε||500A2||IHC-F|
|Alexa Fluor® 488 anti-mouse CD3ε||500A2||FC,IHC-F,3D IHC|
|APC/Cyanine7 anti-mouse CD3ε||500A2||FC|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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APC anti-mouse CD3ε
FITC anti-mouse CD3ε
PerCP/Cyanine5.5 anti-mouse CD3ε
PE/Cyanine7 anti-mouse CD3ε
PE anti-mouse CD3ε
APC/Fire™ 750 anti-mouse CD3ε
Alexa Fluor® 700 anti-mouse CD3ε
Purified anti-mouse CD3ε
Alexa Fluor® 594 anti-mouse CD3ε
Alexa Fluor® 647 anti-mouse CD3ε
Alexa Fluor® 488 anti-mouse CD3ε
APC/Cyanine7 anti-mouse CD3ε