- BC96 (See other available formats)
- Regulatory Status
- V T-072
- Other Names
- Low affinity IL-2R, IL-2R α chain, Tac, p55
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.Product Details
- Verified Reactivity
- Reported Reactivity
- Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The CD25 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Blue Laser (488 nm)
- Application Notes
Additional reported applications include: immunocytochemistry3.
- Application References
- Product Citations
AB_493044 (BioLegend Cat. No. 302615)
AB_493043 (BioLegend Cat. No. 302616)
- Type I transmembrane glycoprotein, 55 kD
Activated T cells and B cells, monocytes/macrophages, Treg
- Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
- Cell Type
- B cells, Macrophages, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681.
- Gene ID
- 3559 View all products for this Gene ID
- View information about CD25 on UniProt.org
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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APC anti-human CD25
FITC anti-human CD25
PE anti-human CD25
PE/Cyanine5 anti-human CD25
Purified anti-human CD25
APC/Cyanine7 anti-human CD25
PE/Cyanine7 anti-human CD25
Alexa Fluor® 488 anti-human CD25
Alexa Fluor® 647 anti-human CD25
Pacific Blue™ anti-human CD25
Alexa Fluor® 700 anti-human CD25
Biotin anti-human CD25
PerCP/Cyanine5.5 anti-human CD25
Brilliant Violet 421™ anti-human CD25
Brilliant Violet 605™ anti-human CD25
Brilliant Violet 650™ anti-human CD25
Brilliant Violet 711™ anti-human CD25
Brilliant Violet 785™ anti-human CD25
Brilliant Violet 510™ anti-human CD25
APC/Fire™ 750 anti-human CD25
TotalSeq™-A0085 anti-human CD25
PE/Dazzle™ 594 anti-human CD25
TotalSeq™-B0085 anti-human CD25
TotalSeq™-C0085 anti-human CD25
TotalSeq™-D0085 anti-human CD25