- L5 (See other available formats)
- Other Names
- FLAG tag
- Rat IgG2a, λ
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- Product Citations
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The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.Product Details
- DYKDDDDK tag epitope
- Antibody Type
- Host Species
- DYKDDDDK-tagged mouse Langerin
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml (1:250 - 1:1000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Blue Laser (488 nm)
- Application Notes
The L5 clone has been demonstrated to have 2-8 fold better sensitivity in WB than another commonly used antibody clone, M2.
- Application References
- Park SH, et al. 2008. J Immunol Methods. 331:27.
- Moon SH, et al. 2010. J. Biol Chem. 285:12935. PubMed
- Sasaki M, et al. 2011. J. Biol Chem. 286:39370. PubMed
- Sonder SU, et al. 2012. J Immunol. 188:5906. PubMed
- Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
- Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
- Toyo-Oak K, et al. 2014. J Neurosci. 34:12168. PubMed
AB_2728470 (BioLegend Cat. No. 637317)
AB_2810690 (BioLegend Cat. No. 637318)
- Biology Area
- Cell Biology
- Antigen References
1. Einhauer A. 2001. J. Biochem. Biophys. Methods. 49:455.
2. Knappik A and Pluckthun A. 1994. Biotechniques. 17:754.
- Gene ID
- View information about DYKDDDDK on UniProt.org
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