Product: ELISA MAX™ Standard Set Mouse IL-1β |
Catalog No.: 432603 |
Dimura Rasim, Master student University of Pristina |
Mouse IL-1β ELISA | |||
Overall: | Product Quality: | Ease of Use: | |
IL-1β cytokine production |
Experimental Design |
Application: | ELISA |
Cells used: | Mouse monocytes |
Brief Protocol: | Capture antibody to the plate, and 24h incubation. Second day, block, add the samples and then detection antibody. After this add the enzyme and the substrate and stop with an acid. Read the plate. |
Results Summary: | Sensitive detection of IL-1β |
Additional Notes: | Check first the Standard curve concentration range. Some times you need to pre-stimulate the supernatants with ATP due to low production on IL-1beta. |
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