Product: Purified anti-HA.11 Epitope Tag |
Catalog No.: 901501 |
Carter Barger, Graduate Research Assistant University of Nebraska Medical Center |
Anti-HA antibody for Western Blotting | |||
Overall: | Product Quality: | Ease of Use: | |
The antibody was clean and performed very well for the detection of HA-tagged protein expressed in rat cells. |
Experimental Design |
Application: | Western Blotting |
Cells used: | IEC-18 rat intestinal crypt-like cells (ATCC CRL-1589) |
Brief Protocol: | Cells were lysed for total protein with SDS buffer, quantified and loaded for separation on SDS-page gel. Proteins were transferred to a nitrocellulose membrane, blocked in milk and probed as following: anti-HA (1:1000), horseradish peroxidase-conjugated goat anti-mouse IgM (1:2000). |
Results Summary: | Anti-HA detected our tagged recombinant HA protein but did not cross-react with endogenous proteins. |
Additional Notes: |
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