Application: |
Flow Cytometry |
Cells used: |
Human PBMC |
Brief Protocol: |
500K PBMC freshly isolated were stained with an antibody cocktail containing CCR6 Brilliant Violet 605™(clone G034E3) for 15min at RT at dark in a 96V plate. Dilution used 3/100 from working antibody concentration of 20ug/ml. Cells were then washed twice and span at 2000 rpm for 4 min and acquired on LSRII. |
Results Summary: |
Bright antibody for nice TH or TfH separation. |
Additional Notes: |
Stock concentration of 100ug/ml was diluted to a working concentration of 20ug/ml for a final concentration of 1ug/ml during staining. |
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