Product: Monensin Solution (1,000X) |
Catalog No.: 420701 |
Romy Hackbusch, Miss University Medical Center Hamburg-Eppendorf |
Monensin Solution 1000x | |||
Overall: | Product Quality: | Ease of Use: | |
Monensin works well for inhibition of protein transport. |
Experimental Design |
Application: | Flow Cytometry |
Cells used: | Human Whole Blood |
Brief Protocol: | Monensin is used in a final concentration of 2mM. Stimulate whole blood in the presence of Monensin for 5 hours, followed by washing, staining surface markers, fixation, permeabilization and staining cytokines. |
Results Summary: | Monensin inhibits the protein transport very well and is absolutely advisable for ICS. |
Additional Notes: | Store at +4°C. |
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