Product: Purified anti-Tubulin β 3 (TUBB3)

Catalog No.: 801201

Lori OBrien, Assistant Professor UNC-Chapel Hill

Purified anti-Tubulin β 3 (TUBB3)
Overall:	Product Quality:	Ease of Use:
Excellent, specific staining on mouse tissue.

Experimental Design

Application:	Whole mount fluorescent immuno and cryo section immune
Cells used:	Mouse embryo (E11.5) and embryonic kidney (E15.5)
Brief Protocol:	No antigen retrieval necessary Block 20 min-1 hour donkey serum (section) or sheep serum (whole mount) O/N primary incubation for whole mount (1:250 anti-Tuj1) or 1-2 hr incubation for section (1:1000) O/N secondary incubation for whole mount (1:250 Alexa Fluor 488 donkey anti-mouse IgG2a ) or 1 hr incubation for section (1:1000 ) Whole mount tissue cleared with iDISCO+
Results Summary:	Excellent, specific staining with no background. Antibody holds up to iDISCO+ tissue clearing and gave great signal on light sheet microscope.
Additional Notes:

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Publication Status This data has not been published.

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