Application: |
Flow Cytometry |
Cells used: |
Mouse Draining Lymph Node |
Brief Protocol: |
Draining lymph nodes following immunization were isolated and filtered to a single cell suspension. Cells had Fc receptors blocked. Cells were then stained for 30 minutes on ice with 1:200 CD4, 1:100 anti-CXCR5, and 1:250 BV421 anti-mouse PD1. Cells were washed 2 times with FACs buffer. Cells were then fixed and data was analyzed on an LSR Fortessa. |
Results Summary: |
Follicular helper T cells were successfully identified using BV421 anti-mouse PD1. |
Additional Notes: |
2D plot is gated on CD4+ T cells. |
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