Product: Brilliant Violet 711™ anti-mouse/human CD11b

Catalog No.: 101242

karine Serre, PhD Instituto de Medicina Molecular - iMM

Brilliant Violet 711™ anti-mouse CD11b Antibody
Overall:	Product Quality:	Ease of Use:
Anti-mouse CD11b BV711™ brightly stains myeloid cells.

Experimental Design

Application:	Flow Cytometry
Cells used:	Cell suspensions from mouse lymphoid tissues such as spleen and lymph nodes, (but also peripheral non-lymphoid tissues such as brain, lung, liver, and tumour).
Brief Protocol:	We isolated cells from peripheral lymphoid tissues, spleen and lymph nodes, from C57BL/6 mice, and stained them with anti-mouse CD11b-BV711™ antibody. We usually put up to 5 x 10^6 cells in a 50ul volume and add anti-mouse CD11b-BV711™ diluted at 1/1000 final (excellent antibody!!) for 1h at room temperature.
Results Summary:	Anti-mouse CD11b-BV711™ antibody provides a strong signal that allows reliable staining to study myeloid cells from various types of mouse tissues.
Additional Notes:	Our laboratory has a long lasting interest in immune responses and we have tested many different fluorescent antibodies to detect myeloid cell populations. We choose to use routinely anti-mouse CD11b-BV711™ as this was the best for our combination with more 9 other colours. Figure shows anti-CD11b BV711™ staining from total splenocytes of C57BL6 mouse.

Data Image Click on the image for full size data.

Publication Status This data has not been published.

See all Reviews