Product: PerCP/Cyanine5.5 anti-mouse CD3ε |
Catalog No.: 100328 |
Andreia Lino, PhD Deutsches Rheuma-Forschungszentrum, a Leibniz Institute |
Anti-mouse CD3 PerCP/Cyanine5.5 for Flow Cytometry | |||
Overall: | Product Quality: | Ease of Use: | |
Good, reliable and not very bright. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Mouse splenocytes |
Brief Protocol: | Resuspend in PBS/BSA and count the cells. Load up to 5x106 cells onto a V-Bottom 96-well-plate. Spin down, resuspend in 50uL of PBS/BSA containing αFcγR (24.62) 30 µg/ml and incubate 15min at 4°C. Spin down and resuspend in 50 µl antibody staining solution containing anti-CD3 PerCP/Cyanine5.5 (1:100). Incubate 15min at 4°C in the dark. Wash twice with PBS/BSA, resuspend and transfer to FACS tubes to read. |
Results Summary: | Approximately half of the live lymphocytes in the spleen should be CD3 PerCP/Cyanine5.5 + (T cells). |
Additional Notes: |
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