Product: PE/Cyanine7 anti-mouse CD169 (Siglec-1) |
Catalog No.: 142411 |
Nathan Jacobs, PhD Candidate Emory University |
Easy Identification of CD169+ Macrophages (PE/Cyanine7 anti-mouse) | |||
Overall: | Product Quality: | Ease of Use: | |
CD169+ cells are rare, but this antibody makes finding them a breeze. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Lungs, Splenocytes |
Brief Protocol: | Spleens were homogenized and RBC lysed before staining. Lungs were chopped and digested with collagenase D and DNAse II, then leukocytes were purified by Percoll gradient before staining. Cells were Fc blocked for 20min @ RT, then stained for 30min @ 4ºC, then washed 3x before analysis on a BD LSR-II. |
Results Summary: | Nice clear staining of CD169. |
Additional Notes: |
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