Application: |
Flow Cytometry |
Cells used: |
Mouse T cells |
Brief Protocol: |
Followed BioLegend's recommended dilution. It was incubated in eBioscience permeabilization buffer. I followed the fix/perm protocol from eBioscience. |
Results Summary: |
For this experiment, I fixed and perm my cells. For compensation, I used eBioscience's beads. This antibody was a poor antibody to detect Foxp3. |
Additional Notes: |
When I was acquiring my experimental sample, most of the events was not showing within the dot plot and had to recompensate and alter the PMT voltage. A few scattered events were displaying in the Foxp3 positive gate, however I question the validity of the results. |
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