Make your data DAZZLE.
Learn more about BioLegend's newest tandem, PE/Dazzle™ 594.
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Introduction
BioLegend is proud to unveil our newest tandem fluorophore, PE/Dazzle™ 594. PE/Dazzle™ 594 provides a great new option to expand your multicolor panel, a bright signal to detect your target antigen with little background noise, and excellent pricing. PE/Dazzle™ 594 is an equivalent fluorophore to PE/Texas Red®, PE-CF594, PE-eFluor® 610, and ECD.
PE/Dazzle™ 594 offers:
- Outstanding brightness
- Outstanding pricing
- Lower compensation requirements compared to spectrally equivalent fluorophores
- Exceptional photostability
- Multicolor panel design flexibility
- If your desired antibody is not available, then request a custom conjugation.
Excitation & Emission Spectra
PE/Dazzle™ 594 excites optimally at 566 nm and emits optimally at 610 nm. PE/Dazzle™ 594 can be excited by the blue (488 nm), green (532 nm) or yellow/green lasers (561 nm), and is detected in the PE/Texas Red® channel. A common filter for this channel is a 610/20 bandpass.
Compare PE/Dazzle™ 594 against other fluors with our Fluorescence Spectra Analyzer.
Data
Human peripheral blood lymphocytes were stained with anti-human CD3 (clone UCHT1) conjugated to either PE/Dazzle™ 594 (purple) or other companies’ equivalent fluorophore (blue or red).
Human peripheral blood lymphocytes were stained with FITC anti-human CD3 (clone UCHT1) and anti-human CD56 conjugated to either PE/Dazzle™ 594 or other companies’ equivalent fluorophore.
PE/Dazzle™ 594 provides excellent resolution of your populations.
Human peripheral blood lymphocytes were stained with APC anti-human CD11c (clone 3.9) and PE/Dazzle™ 594 anti-human CD86 (clone IT2.2).
Human peripheral blood lymphocytes were stained with APC anti-human CD4 (clone RPA-T4) and PE/Dazzle™ 594 anti-human CD25 (clone M-A251).
The Tandem
The LEGENDeers have released their new hit single "My Tandem" which was featured in the finale.
Discover how PE met his better half from the very beginning with our video series, The Tandem. And, learn more about tandem dye pairings here.
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