Western Blot


Direct-Blot™ HRP Antibodies
Western-Ready™ ECL Substrate Kit
Prime Step™ Protein Ladder
Primary Antibodies for Western Blot
Secondary Reagents for Western Blot
Western Blot Controls
Western Blot Sampler Kits

IHC/ICC


Primary Antibodies for IHC
Primary Antibodies for ICC
Secondary Reagents for IHC/ICC
Chemical Probes

 

All Applications


Antibodies for Phospho-Proteins
KO/KD Validated Antibodies

IP/ChIP 


Go-ChIP-Grade™ Products
Antibodies for IP
Affinity Gels for IP/Purification
Epitope Tag Antibodies

 

Other Applications


Flow Cytometry
ELISA and Multiplex
Cell Function and Viability Assay Kits

Application Protocols

Western Blot

IHC/ICC

IP/ChIP


BioLegend’s Commitment to Antibody Quality

 

To ensure the highest quality of products, BioLegend’s cell biology antibodies undergo extensive validation processes, which include:

 

  • Testing on knockout (KO) or knockdown (KD) cell lysates (CRISPR/Cas9 or siRNA), and negative control cell lines to confirm antibody specificity.
  • Testing on multiple species (human, mouse, rat, yeast, bacteria, zebrafish) whenever applicable, to determine antibody cross-reactivity.
  • Side-by-side comparison testing with competitor’s antibody to ensure sensitivity.
  • Side-by-side comparison testing of lots with internal controls for lot-to-lot consistency.
  • Clones for one application (such as WB or ChIP) further validated in multiple applications (such as microscopy, flow cytometry) to provide the user the flexibility of supplementing their data with additional assays specific to their research.
  • Optimization of antibody suggested use by a titration range.
validation image

Learn more about BioLegend's Reproducibility and Validation Initiatives, and how you can improve your own reproducibility.