Application Protocols

To ensure the highest quality of products, BioLegend’s cell biology antibodies undergo extensive validation processes, which include:

• Testing on knockout (KO) or knockdown (KD) cell lysates (CRISPR/Cas9 or siRNA), and negative control cell lines to confirm antibody specificity.
• Testing on multiple species (human, mouse, rat, yeast, bacteria, zebrafish) whenever applicable, to determine antibody cross-reactivity.
• Side-by-side comparison testing with competitor’s antibody to ensure sensitivity.
• Side-by-side comparison testing of lots with internal controls for lot-to-lot consistency.
• Clones for one application (such as WB or ChIP) further validated in multiple applications (such as microscopy, flow cytometry) to provide the user the flexibility of supplementing their data with additional assays specific to their research.
• Optimization of antibody suggested use by a titration range.

Learn more about BioLegend's Reproducibility and Validation Initiatives, and how you can improve your own reproducibility.