B cells are involved in a number of immune functions, including antibody generation. Given their importance in B cell lymphomas and some autoimmune diseases, the depletion of B cells with monoclonal antibodies is an essential FDA approved treatment, as well as a very active field of research. In particular, CD20, which aids in calcium influx, activation and proliferation of B cells, can be found on pre-B cells and the stages of differentiation that follow (with the exception of plasma cells). BioLegend is proud to announce the release of a new clone, SA271G2, which exhibits the ability to deplete murine B cells in vivo, providing a valuable research tool for investigators.

In vivo depletion protocol

C57BL/6 mice were injected i.v. with 250 µg of Ultra-LEAF™ purified mAb SA271G2 or Ultra-LEAF™ purified rat IgG2b, κ isotype control. To study the presence of B cells in different compartments, the mice were either bled or euthanized with CO2 at day 7, and spleen, lymph nodes, bone marrow, and the peritoneal cavity lavage were collected and stained with the indicated antibodies.

Spleen sections demonstrate loss of B cells with administration of clone SA271G2

C57BL/6 mice were injected i.v. with 250 µg of purified rat IgG2b, κ isotype control (left) or Ultra-LEAF™ purified anti-mouse CD20 (clone SA271G2) (right). At day 7, Immunofluorescence staining of spleen frozen sections shows depletion of the different B cell subsets in mice that were injected with clone SA271G2. T cells, macrophage subsets, and overall architecture of the spleen are not affected by SA271G2 treatment.

B cells are depleted from mouse blood using clone SA271G2

C57BL/6 mice were injected i.v. with 250 µg of purified rat IgG2b, κ isotype control (left) or Ultra-LEAF™ purified anti-mouse CD20 (clone SA271G2) (right). At day 7 the mice were bled and the samples were stained with anti-mouse CD19 APC and anti-mouse CD45 Brilliant Violet 421™. Data shown is representative of 4 mice per group.

Immature B cell-specific depletion in the bone marrow

In vivo administration of mAb SA271G2 depletes immature stage bone marrow. Bone marrow cells from SA271G2-treated mice or the isotype-treated control group were stained to identify the different B cell subsets. Only the immature B cells (CD19hi B220hi) were depleted, but not pre-pro, pro, or pre-B cells. Data shown was gated on the lymphoid population.

Anti-Mouse CD20 Antibody (Clone SA271G2) and Isotype Control

Description
Ultra-LEAF™ Purified anti-mouse CD20 Antibody
Ultra-LEAF™ Purified Rat IgG2b, κ Isotype Ctrl Antibody

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