Instructions for Use
BioLegend's Fluorescence Spectra Analyzer is useful for the analysis of excitation and emission spectra of commonly used fluorochromes for flow cytometry.

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1. View Spectra
Normalized emission curves are plotted into each channel off the ultraviolet, violet, blue, yellow/green, and red lasers of Cytek™ Aurora flow cytometers. You can uncheck and remove lasers as necessary to better match your instrument. To view spectra, simply select a fluorophore from one of the dropdowns and then check or uncheck the box to toggle it on or off. The displayed emission spectrum color for each fluorophore is indicated to the right of its name.
2. Customize Colors
All the colors for spectra, laser lines, and filters are customizable. After you have made your selections, click the Customize Colors button to open the interface panel. To change colors, click on the desired color box. Select colors by dragging the locator within the box gradient and/or use the color slider. You can also enter in RGB values if known. To go back to the default color, click on the top right box which shows the default color.
3. Export Image
To export and save the data as an image, click the Export Spectra button. A new window with the data will appear as a PNG file. Save it by right clicking and select “Save image as” or select “Copy image” to paste into another image editor.
  Custom Color Panel

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