Western-Ready™ Transfer Buffer (10X)

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426313 1 L 48€
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Technical Data Sheet (pdf)

Product Details

This buffer is bottled as 10X. To prepare a working solution (1X) dilute Western-Ready™ Transfer Buffer (10X) to 1X concentration (1:10 by volume). 1 part of Western-Ready™ Transfer Buffer (10X), 2 parts of 100% methanol, and 7 parts of DI water.
Storage & Handling
Upon receipt, store solutions at room temperature. Products are shipped at ambient (room) temperature.

WB - Quality tested

Recommended Usage

Dilute Western-Ready Transfer Buffer (10X) to 1X concentration (1:10 by volume). 1 part of Western-Ready Transfer Buffer (10X), 2 parts of 100% methanol, and 7 parts of DI water.

Application Notes

This buffer is formulated for Western blot protein transfer. By direct PDVF membrane staining using Licor Revert 700 protein dye, we are able to detect as low as 25 ng/band on high and medium molecular weight proteins, and as low as 12.5 ng/band in low molecular weight proteins. The buffer is validated for protein transfer to both nitrocellulose and PVDF membranes. This buffer is only recommended for wet  protein transfers.

Western-Ready Transfer Buffer does not include any methanol.

Antigen Details

Gene ID

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Go To Top Version: 1    Revision Date: 05/04/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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