Ultra-LEAF™ Purified anti-CCL11 Antibody

Pricing & Availability
Clone
L403H11 (See other available formats)
Other Names
Eotaxin, eotaxin-1, small inducible cytokine subfamily A, member 11 (SCYA11)
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
L403H11_PURE_CCL11_ELISA_Antibody_122016
Ultra-LEAF™ Purified anti-mouse CCL11 Antibody inhibits the chemotaxis induced by mouse CCL11 (30 ng/ml) on mouse CCR3 transfected BA/F3 mouse cells in a dose dependent manner, with an ED50= 0.25 – 1.25 µg/ml.
  • L403H11_PURE_CCL11_ELISA_Antibody_122016
    Ultra-LEAF™ Purified anti-mouse CCL11 Antibody inhibits the chemotaxis induced by mouse CCL11 (30 ng/ml) on mouse CCR3 transfected BA/F3 mouse cells in a dose dependent manner, with an ED50= 0.25 – 1.25 µg/ml.
  • L403H11_ULEAF_CCL11_Antibody_022616
    Ultra-LEAF™ Purified anti-mouse CCL11 Antibody inhibits the chemotaxis induced by mouse CCL11 (30 ng/ml) on mouse CCR3 transfected BA/F3 mouse cells in a dose dependent manner, with an ED50= 0.25 – 1.25 µg/ml.
Cat # Size Price Quantity Avail. Save
683203 500 µg 396€
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Description

CCL11 is a member of the CC chemokine family and was originally identified as a chemoattractant for eosinophils. It participates in a variety of allergic diseases in response to allergens. CCL11 induces angiogenic response by CCR3-positive microvascular endothelial cells. Also, CCL11 facilitates proliferation and metastasis of tumor cells expressing CCR3.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Mouse CCL11, amino acids (His24-Pro97) (Accession# NM_011330), was expressed in E. coli.
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
ELISA Capture, Neut - Validated

Recommended Usage

Each lot of this antibody is quality control tested by blocking chemotaxis induced by mouse CCL11 (30 ng/ml) on mouse CCR3 transfected BA/F3 mouse cells. The ED50 is 0.25 - 1.25 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For highly sensitive assays, we recommend Ultra-Leaf™ purified antibody (Cat. No. 683203) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

RRID
AB_2571917 (BioLegend Cat. No. 683203)

Antigen Details

Structure
Chemokine.
Distribution

Epithelial cells, smooth muscle cells, dendritic cells, fibroblasts, mast cells, and Th2 lymphocytes.

Function
Recruits eosinophils in allergic responses. Stimulates eosinophil release from bone marrow, increasing levels of circulating eosinophils. Induced by pro-inflammatory cytokines (IL-4, IL-13, TNF-7α, and IFN-α).
Interaction
Eosinophils.
Ligand/Receptor
CCR3.
Cell Type
Epithelial cells, Dendritic cells, Fibroblasts, Mast cells, Th2
Biology Area
Angiogenesis, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Crump MP, et al. 1998. J. Biol. Chem. 273:22471.
2. Conroy DM, et al. 2001. Respir. Res. 2:150.
3. Salcedo R, et al. 2001. J. Immunol. 166:7571.
4. Hadjicharalambous C, et al. 2004. J. Allergy Clin. Immunol. 113:657.
5. Kaehler J, et al. 2006. J. Investig. Med. 54:446.
6. Levina V, et al. 2009. Clin. Cancer Res. 15:2647.

Gene ID
20292 View all products for this Gene ID
UniProt
View information about CCL11 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 12/20/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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