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Ultra-LEAF™ Purified anti-CCL11 Antibody

Pricing & Availability
Clone
L403H11 (See other available formats)
Regulatory Status
RUO
Other Names
Eotaxin, eotaxin-1, small inducible cytokine subfamily A, member 11 (SCYA11)
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
L403H11_PURE_CCL11_ELISA_Antibody_122016
Ultra-LEAF™ Purified anti-mouse CCL11 Antibody inhibits the chemotaxis induced by mouse CCL11 (30 ng/ml) on mouse CCR3 transfected BA/F3 mouse cells in a dose dependent manner, with an ED50= 0.25 – 1.25 µg/ml.
  • L403H11_PURE_CCL11_ELISA_Antibody_122016
    Ultra-LEAF™ Purified anti-mouse CCL11 Antibody inhibits the chemotaxis induced by mouse CCL11 (30 ng/ml) on mouse CCR3 transfected BA/F3 mouse cells in a dose dependent manner, with an ED50= 0.25 – 1.25 µg/ml.
  • L403H11_ULEAF_CCL11_Antibody_022616
    Ultra-LEAF™ Purified anti-mouse CCL11 Antibody inhibits the chemotaxis induced by mouse CCL11 (30 ng/ml) on mouse CCR3 transfected BA/F3 mouse cells in a dose dependent manner, with an ED50= 0.25 – 1.25 µg/ml.
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683203 500 µg 436€
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Description

CCL11 is a member of the CC chemokine family and was originally identified as a chemoattractant for eosinophils. It participates in a variety of allergic diseases in response to allergens. CCL11 induces angiogenic response by CCR3-positive microvascular endothelial cells. Also, CCL11 facilitates proliferation and metastasis of tumor cells expressing CCR3.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Mouse CCL11, amino acids (His24-Pro97) (Accession# NM_011330), was expressed in E. coli.
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
ELISA Capture, Neut - Verified
Recommended Usage

Each lot of this antibody is quality control tested by blocking chemotaxis induced by mouse CCL11 (30 ng/ml) on mouse CCR3 transfected BA/F3 mouse cells. The ED50 is 0.25 - 1.25 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For highly sensitive assays, we recommend Ultra-Leaf™ purified antibody (Cat. No. 683203) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

RRID
AB_2571917 (BioLegend Cat. No. 683203)

Antigen Details

Structure
Chemokine.
Distribution

Epithelial cells, smooth muscle cells, dendritic cells, fibroblasts, mast cells, and Th2 lymphocytes.

Function
Recruits eosinophils in allergic responses. Stimulates eosinophil release from bone marrow, increasing levels of circulating eosinophils. Induced by pro-inflammatory cytokines (IL-4, IL-13, TNF-7α, and IFN-α).
Interaction
Eosinophils.
Ligand/Receptor
CCR3.
Cell Type
Epithelial cells, Dendritic cells, Fibroblasts, Mast cells, Th2
Biology Area
Angiogenesis, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Crump MP, et al. 1998. J. Biol. Chem. 273:22471.
2. Conroy DM, et al. 2001. Respir. Res. 2:150.
3. Salcedo R, et al. 2001. J. Immunol. 166:7571.
4. Hadjicharalambous C, et al. 2004. J. Allergy Clin. Immunol. 113:657.
5. Kaehler J, et al. 2006. J. Investig. Med. 54:446.
6. Levina V, et al. 2009. Clin. Cancer Res. 15:2647.

Gene ID
20292 View all products for this Gene ID
UniProt
View information about CCL11 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CCL11 Reagents Request Custom Conjugation
Description Clone Applications
Ultra-LEAF™ Purified anti-CCL11 L403H11 Block,ELISA Capture,Neut
Purified anti-CCL11 L403H11 ELISA Capture
Go To Top Version: 2    Revision Date: 12/20/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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